https://scholars.lib.ntu.edu.tw/handle/123456789/562474
標題: | Comparison of 2 interferon-γ assays and Roche Cobas Amplicor Mycobacterium tuberculosis assay for rapid diagnosis of tuberculosis among patients with suspected tuberculosis in Taiwan | 作者: | Chou C.-H. Hsu H.-L. LI-NA LEE PO-REN HSUEH KWEN-TAY LUH |
公開日期: | 2009 | 卷: | 42 | 期: | 3 | 起(迄)頁: | 251-257 | 來源出版物: | Journal of Microbiology, Immunology and Infection | 摘要: | Background and purpose: Two commercial interferon-γ (IFN-γ) assays, which are commonly used for diagnosing latent tuberculosis (TB), are also useful for diagnosis of active TB. In this study, the IFN-γ assays and polymerase chain reaction (PCR) for diagnosis of TB were compared. Methods: A prospective comparison of the performance of 2 commercial IFN-γ assays - QuantiFERON?-TB Gold (QFT-G) and T-SPOT?.TB (T SPOT) - and PCR using the Roche Cobas Amplicor Mycobacterium tuberculosis (RCA-TB) assay for the rapid diagnosis of TB was conducted from January 2007 to December 2007 at a university-affiliated hospital in Taiwan. Results: Of 187 patients enrolled in the study, results from both T SPOT and QFT-G were available for 154, including 109 patients with active TB and 45 with no TB. The sensitivity of T SPOT (89.0%) was higher than that of QFT-G (71.4%). RCA-TB had the highest sensitivity (90.2%) and specificity (100%), but was usually performed in patients with positive acid-fast bacilli smear test. In patients with extrapulmonary TB, T SPOT had a high diagnostic value (sensitivity, 81.3%). A significant discordance between the 2 IFN-γ assays was also noted. IFN-γ assays provided a more rapid diagnosis for tuberculosis than the conventional culture method (mean ± standard deviation, 8.23 ± 12.86 days; p < 0.001). Conclusions: Use of IFN-γ may shorten the time to diagnosis of TB, especially for smear-negative patients and those with extrapulmonary disease. ? 2009 Journal of Microbiology, Immunology and Infection. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/562474 | ISSN: | 1684-1182 | SDG/關鍵字: | alpha2 interferon; adult; article; controlled study; diagnostic value; female; human; intermethod comparison; major clinical study; male; Mycobacterium tuberculosis; polymerase chain reaction; sensitivity and specificity; Taiwan; tuberculosis; Adolescent; Adult; Aged; Aged, 80 and over; Chi-Square Distribution; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Female; Humans; Infant; Interferon-gamma; Male; Middle Aged; Mycobacterium tuberculosis; Polymerase Chain Reaction; Predictive Value of Tests; Prospective Studies; Sensitivity and Specificity; Taiwan; Tuberculosis |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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