https://scholars.lib.ntu.edu.tw/handle/123456789/564856
標題: | Induction of endoplasmic reticulum stress and apoptosis by a marine prostanoid in human hepatocellular carcinoma | 作者: | Chiang P.-C. CHUNG-LIANG CHIEN Pan S.-L. WEN-PIN CHEN Teng C.-M. Shen Y.-C. JIH-HWA GUH |
公開日期: | 2005 | 卷: | 43 | 期: | 4 | 起(迄)頁: | 679-686 | 來源出版物: | Journal of Hepatology | 摘要: | Background/Aims: Hepatocellular carcinoma is a very common malignancy and is highly chemoresistant to currently available chemotherapeutic agents. We isolated a marine prostanoid, bromovulone III, from soft coral Clavularia viridis and found that it displayed effective anti-tumor activity in human hepatocellular carcinoma. The anti-tumor mechanism has been delineated in this study. Methods: Anti-tumor efficacy and apoptotic cell death were examined by sulforhodamine B and Hoechst 33342 assays. Rhodamine 123 was used to measure the change of mitochondrial membrane potential. Immunoprecipitation and Western blotting detect the involvement of several apoptosis-related proteins. Electron microscopic examination detects the morphological change of mitochondria and endoplasmic reticulum (ER). Results: Bromovulone III primarily induced mitochondria-related activation of caspase-9 and -3 in several tumor types, such as prostate cancer PC-3 and acute promyelocytic leukemia HL-60 cells. However, it primarily induced the activation of m-calpain, caspase-12, and transcription factor CHOP/GADD153 in hepatocellular carcinoma Hep3B cells, suggesting the involvement of ER stress. Furthermore, a secondary mitochondrial swelling and depolarization of mitochondrial membrane potential were subsequently triggered after ER stress, suggesting the crosstalk between ER and mitochondria. Conclusions: It is suggested that bromovulone III induces apoptosis in Hep3B cells through a mechanism that induces ER stress and leads to activation of CHOP/GADD153 and caspase-12. ? 2005 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-24344433558&doi=10.1016%2fj.jhep.2005.02.049&partnerID=40&md5=9ecf8ec05fc9173f45b0713b5b21015f https://scholars.lib.ntu.edu.tw/handle/123456789/564856 |
ISSN: | 1688278 | DOI: | 10.1016/j.jhep.2005.02.049 | SDG/關鍵字: | bromovulone iii; calpain; caspase 12; caspase 3; caspase 9; growth arrest and DNA damage inducible protein 153; hoe 33342; prostanoid; rhodamine 123; sulforhodamine B; unclassified drug; antineoplastic activity; apoptosis; article; cancer cell culture; clavularia viridis; controlled study; coral; depolarization; drug efficacy; electron microscopy; endoplasmic reticulum; enzyme activation; human; human cell; immunoprecipitation; liver cell carcinoma; liver mitochondrion; membrane potential; mitochondrial membrane; mitochondrion swelling; priority journal; promyelocytic leukemia; prostate cancer; signal transduction; Western blotting; Apoptosis; Carcinoma, Hepatocellular; Cell Line, Tumor; Endoplasmic Reticulum; Humans; Liver Neoplasms; Oxidative Stress; Prostaglandins |
顯示於: | 藥學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。