https://scholars.lib.ntu.edu.tw/handle/123456789/564859
標題: | A potential role of YC-I on the inhibition of cytokine release in peripheral blood mononuclear leukocytes and endotoxemic mouse models | 作者: | Pan S.-L. JIH-HWA GUH Peng C.-Y. Chang Y.-L. Cheng F.-C. Chang J.-H. Kuo S.-C. Lee F.-Y. Teng C.-H. |
公開日期: | 2005 | 卷: | 93 | 期: | 5 | 起(迄)頁: | 940-948 | 來源出版物: | Thrombosis and Haemostasis | 摘要: | To evaluate the anti-sepsis potential of YC-1, we have examined the effect of YC-1 on the regulation of cytokine production in human leukocytes and endotoxemic mice. The data demonstrated that YC-1 showed a preferential inhibition on proinflammatory cytokine production without inhibition of cell growth or induction of cytotoxicity in human leukocytes. On the other hand, in the septic mouse model, treatment with an intraperitoneal application of LPS caused a cumulative death within 27 hours. The post-treatment administration of YC-1 significantly increased the survival rate in endotoxemic mice. Furthermore, several mediators were detected and the data showed that YC-1 profoundly blocked LPS-induced NO as well as TNF-α production, and prevented lung damage by histological examination. Samples from the animal model showed that LPS-induced NF-κB/DNA binding activity and consequent up-regulation of iNOS expression in tissues were abolished by post-administration of YC-1. Furthermore, YC-1, by itself, did not modify cGMP content while significantly inhibit LPS-induced cGMP formation, suggesting that YC-1-mediated effect was not through a cGMP-elevating pathway.Taken together, it is evident that the post-treatment administration of YC-1 after LPS application significantly inhibits NF-κB activation, iNOS expression, NO over-production, and cytokine release reaction resulting in an improved survival rate in endotoxemic mice. It is suggested that YC-1 may be a potential agent for the therapeutic treatment of sepsis. ? 2005 Schattauer GmbH, Stuttgart. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-18844366701&doi=10.1160%2fTH04-03-0195&partnerID=40&md5=79c708b0d34480f528ed37ccfbde768f https://scholars.lib.ntu.edu.tw/handle/123456789/564859 |
ISSN: | 3406245 | DOI: | 10.1160/TH04-03-0195 | SDG/關鍵字: | 1 benzyl 3 (5 hydroxymethyl 2 furyl)indazole; 1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one; concanavalin A; cyclic GMP; cytokine; DNA; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; lipopolysaccharide; nitric oxide; animal experiment; animal model; animal tissue; article; controlled study; cytokine production; cytotoxicity; drug mechanism; endotoxemia; human; human cell; inflammation; lung injury; male; mouse; nonhuman; peripheral blood mononuclear cell; priority journal; protein expression; sepsis; survival rate; Animals; Blotting, Western; Cyclic GMP; Cytokines; Disease Models, Animal; DNA; Endotoxemia; Humans; Immunohistochemistry; Indazoles; Leukocytes; Leukocytes, Mononuclear; Lipopolysaccharides; Lung; Male; Mice; Mice, Inbred ICR; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase; Nitric Oxide Synthase Type II; Platelet Aggregation Inhibitors; Rats; Rats, Wistar; Shock, Septic; Time Factors; Up-Regulation |
顯示於: | 藥學系 |
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