https://scholars.lib.ntu.edu.tw/handle/123456789/565111
標題: | SCUBE3 is an endogenous TGF-Β receptor ligand and regulates the epithelial-mesenchymal transition in lung cancer | 作者: | Wu Y.-Y. Peck K. YIH-LEONG CHANG SZU-HUA PAN Cheng Y.-F. Lin J.-C. Yang R.-B. Hong T.-M. PAN-CHYR YANG |
公開日期: | 2011 | 卷: | 30 | 期: | 34 | 起(迄)頁: | 3682-3693 | 來源出版物: | Oncogene | 摘要: | Signal peptide-CUB-EGF-like domain-containing protein 3 (SCUBE3) is a secreted glycoprotein that is overexpressed in lung cancer tumor tissues and is correlated with the invasive ability in a lung cancer cell line model. These observations suggest that SCUBE3 may have a role in lung cancer progression. By exogenous SCUBE3 treatment or knockdown of SCUBE3 expression, we found that SCUBE3 could promote lung cancer cell mobility and invasiveness. Knockdown of SCUBE3 expression also suppressed tumorigenesis and cancer metastasis in vivo. The secreted SCUBE3 proteins were cleaved by gelatinases (matrix metalloprotease-2 (MMP-2) and MMP-9) in media to release two major fragments: the N-terminal epidermal growth factor-like repeats and the C-terminal complement proteins C1r/C1s, Uegf and Bmp1 (CUB) domain. Both the purified SCUBE3 protein and the C-terminal CUB domain fragment, bound to transforming growth factor-Β (TGF-Β) type II receptor through the C-terminal CUB domain, activated TGF-Β signaling and triggered the epithelial-mesenchymal transition (EMT). This process includes the induction of Smad2/3 phosphorylation, the increase of Smad2/3 transcriptional activity and the upregulation of the expression of target genes involved in EMT and cancer progression (such as TGF-Β1, MMP-2, MMP-9, plasminogen activator inhibitor type-1, vascular endothelial growth factor, Snail and Slug), thus promoting cancer cell mobility and invasion. In conclusion, in lung cancer cells, SCUBE3 could serve as an endogenous autocrine and paracrine ligand of TGF-Β type II receptor, which could regulate TGF-Β receptor signaling and modulate EMT and cancer progression. ? 2011 Macmillan Publishers Limited All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-80052062312&doi=10.1038%2fonc.2011.85&partnerID=40&md5=1df79ee1ed73350863f1b3f9e0fe0c52 https://scholars.lib.ntu.edu.tw/handle/123456789/565111 |
ISSN: | 0950-9232 | DOI: | 10.1038/onc.2011.85 | SDG/關鍵字: | epidermal growth factor; gelatinase A; gelatinase B; ligand; plasminogen activator inhibitor 1; procollagen C proteinase; protein scube3; signal peptide; Smad2 protein; Smad3 protein; transcription factor Slug; transcription factor Snail; transforming growth factor beta receptor 2; transforming growth factor beta1; unclassified drug; vasculotropin; amino terminal sequence; animal experiment; animal tissue; article; cancer cell; cancer cell culture; cancer growth; cancer invasion; carboxy terminal sequence; carcinogenesis; cell invasion; cell motility; clinical article; controlled study; epithelial mesenchymal transition; gene silencing; genetic transcription; human; human cell; in vivo study; lung cancer; male; metastasis; mouse; nonhuman; priority journal; protein cleavage; protein domain; protein expression; protein function; protein secretion; regulatory mechanism; signal transduction; upregulation; Calcium-Binding Proteins; Disease Progression; Epithelial-Mesenchymal Transition; Gene Knockdown Techniques; Humans; Ligands; Lung Neoplasms; Neoplasm Invasiveness; Neoplasm Metastasis; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Transforming Growth Factor beta; Gastropoda |
顯示於: | 基因體暨蛋白體醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。