https://scholars.lib.ntu.edu.tw/handle/123456789/565248
標題: | Rapid determination of aristolochic acids I and II in herbal products and biological samples by ultra-high-pressure liquid chromatography-tandem mass spectrometry | 作者: | CHING-HUA KUO Lee C.-W. Lin S.-C. Tsai I.-L. SHOEI-SHENG LEE YUFENG JANE TSENG Kang J.-J. Peng F.-C. Wei-Chu L. |
關鍵字: | Aristolochic acid; Aristolochic acid nephropathy; Biological samples; Herbal products; UHPLC-MS/MS | 公開日期: | 2010 | 卷: | 80 | 期: | 5 | 起(迄)頁: | 1672-1680 | 來源出版物: | Talanta | 摘要: | Aristolochic acids (AAs) are a mixture of structural-related compounds, in which aristolochic acid I (AA I) and aristolochic acid II (AA II) are reported to be correlated with Aristolochic acid nephropathy (AAN). In this work, a rapid and sensitive ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to determine AA I and AA II in herbal products and biological fluids. By using gradient elution with a mobile phase composed of a mixture of 10 mM ammonium formate buffer (pH 3.0) and acetonitrile, AAs could be determined within 10 min. Under optimum UHPLC-MS/MS conditions, the limit of detections was 0.14 and 0.26 ng mL-1 for AA I and AA II, respectively. Run-to-run repeatability and intermediate precision of peak area for AA I and AA II were less than 5.74% relative standard deviation (RSD). Accuracy was tested by spiking 10, 100 and 1000 ng mL-1 in rat serum and the recoveries were within 76.5-92.9%. Matrix effects were within 78.8-127.7%. The developed method was successfully applied to determine AA I and AA II in several herbal products and to investigate their pharmacokinetic behavior in female Wister rats. The result shows that the developed UHPLC-MS/MS method is efficient, sensitive, and accurate for the determination of AA I and AA II in herbal products and biological samples. © 2009 Elsevier B.V. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-75749114019&doi=10.1016%2fj.talanta.2009.10.003&partnerID=40&md5=eedf3b1af953e83fb3ba81d187340b73 https://scholars.lib.ntu.edu.tw/handle/123456789/565248 |
ISSN: | 399140 | DOI: | 10.1016/j.talanta.2009.10.003 | SDG/關鍵字: | Body fluids; High pressure liquid chromatography; Mass spectrometry; Mixtures; Aristolochic acid; Biological samples; Herbal products; Nephropathy; U-HPLC-MS; Ascorbic acid |
顯示於: | 藥學系 |
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