https://scholars.lib.ntu.edu.tw/handle/123456789/565394
標題: | Enhanced radiosensitization for cancer treatment with gold nanoparticles through sonoporation | 作者: | SHAO-LUN LU Liu, W.-W. Cheng, J.C. Lin, L.-C. Wang, C.-R.C. PAI-CHI LI |
公開日期: | 2020 | 出版社: | MDPI AG | 卷: | 21 | 期: | 21 | 起(迄)頁: | 1-16 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | We demonstrate the megavoltage (MV) radiosensitization of a human liver cancer line by combining gold-nanoparticle-encapsulated microbubbles (AuMBs) with ultrasound. Microbubbles- mediated sonoporation was administered for 5 min, at 2 h prior to applying radiotherapy. The intracellular concentration of gold nanoparticles (AuNPs) increased with the inertial cavitation of AuMBs in a dose-dependent manner. A higher inertial cavitation dose was also associated with more DNA damage, higher levels of apoptosis markers, and inferior cell surviving fractions after MV X-ray irradiation. The dose-modifying ratio in a clonogenic assay was 1.56 ± 0.45 for a 10% surviving fraction. In a xenograft mouse model, combining vascular endothelial growth factor receptor 2 (VEGFR2)- targeted AuMBs with sonoporation significantly delayed tumor regrowth. A strategy involving the spatially and temporally controlled release of AuNPs followed by clinically utilized MV irradiation shows promising results that make it worthy of further translational investigations. ? 2020, MDPI AG. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096030169&doi=10.3390%2fijms21218370&partnerID=40&md5=d1e9b17fe89cf4e3543df944fa049279 https://scholars.lib.ntu.edu.tw/handle/123456789/565394 |
ISSN: | 1661-6596 | DOI: | 10.3390/ijms21218370 | SDG/關鍵字: | gold nanoparticle; macrogol; vasculotropin receptor 2; gold; H2AX protein, human; histone; KDR protein, human; metal nanoparticle; vasculotropin receptor 2; animal experiment; animal model; apoptosis; aqueous solution; Article; cancer radiotherapy; cancer recurrence; cancer survival; cancer therapy; clonogenic assay; colony formation; controlled study; DNA damage; double stranded DNA break; genetic marker; Huh-7 cell line; human; human cell; immunofluorescence; in vitro study; in vivo study; inductively coupled plasma mass spectrometry; liver cell carcinoma; male; mass spectrometry; microbubble; mouse; nonhuman; optical density; phase contrast microscopy; radiosensitization; reticuloendothelial system; spectrophotometry; transmission electron microscopy; tumor volume; tumor xenograft; X irradiation; animal; cell survival; devices; drug delivery system; drug screening; liver cell carcinoma; liver tumor; metabolism; microbubble; pathology; procedures; radiation response; radiation tolerance; tumor cell line; ultrasound; Animals; Carcinoma, Hepatocellular; Cell Line, Tumor; Cell Survival; DNA Damage; Drug Delivery Systems; Gold; Histones; Humans; Liver Neoplasms; Metal Nanoparticles; Mice; Microbubbles; Radiation Tolerance; Sonication; Vascular Endothelial Growth Factor Receptor-2; Xenograft Model Antitumor Assays |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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