https://scholars.lib.ntu.edu.tw/handle/123456789/565845
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Jung C.-J. | en_US |
dc.contributor.author | Hsu C.-C. | en_US |
dc.contributor.author | JENG-WEI CHEN | en_US |
dc.contributor.author | Cheng H.-W. | en_US |
dc.contributor.author | CHANG-TSU YUAN | en_US |
dc.contributor.author | YU-MIN KUO | en_US |
dc.contributor.author | RON-BIN HSU | en_US |
dc.contributor.author | JEAN-SAN CHIA | en_US |
dc.date.accessioned | 2021-06-21T01:28:52Z | - |
dc.date.available | 2021-06-21T01:28:52Z | - |
dc.date.issued | 2021-02-01 | - |
dc.identifier.issn | 15537366 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/565845 | - |
dc.description.abstract | Bacterial extracellular DNA (eDNA) and activated platelets have been found to contribute to biofilm formation by Streptococcus mutans on injured heart valves to induce infective endocarditis (IE), yet the bacterial component directly responsible for biofilm formation or platelet adhesion remains unclear. Using in vivo survival assays coupled with microarray analysis, the present study identified a LiaR-regulated PspC domain-containing protein (PCP) in S. mutans that mediates bacterial biofilm formation in vivo. Reverse transcriptase- and chromatin immunoprecipitation-polymerase chain reaction assays confirmed the regulation of pcp by LiaR, while PCP is well-preserved among streptococcal pathogens. Deficiency of pcp reduced in vitro and in vivo biofilm formation and released the eDNA inside bacteria floe along with reduced bacterial platelet adhesion capacity in a fibrinogen-dependent manner. Therefore, LiaR-regulated PCP alone could determine release of bacterial eDNA and binding to platelets, thus contributing to biofilm formation in S. mutans-induced IE. | en_US |
dc.relation.ispartof | PLoS Pathogens | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | chloramphenicol; genomic DNA; spectinomycin; virulence factor; bacterial DNA; bacterial protein; amino acid sequence; Article; bacteremia; bacterial colonization; bacterial endocarditis; bacterial virulence; bacterium identification; biofilm; colony forming unit; endocarditis; immunoprecipitation; microarray analysis; nonhuman; polymerase chain reaction; protein expression; thrombocyte adhesion; animal; extracellular space; genetics; growth, development and aging; host pathogen interaction; human; metabolism; microbiology; normal human; pathology; rat; Streptococcus infection; Streptococcus mutans; thrombocyte adhesion; Animals; Bacterial Proteins; Biofilms; DNA, Bacterial; Endocarditis; Extracellular Space; Healthy Volunteers; Host-Pathogen Interactions; Humans; Platelet Adhesiveness; Rats; Streptococcal Infections; Streptococcus mutans | - |
dc.title | PspC domain-containing protein (PCP) determines Streptococcus mutans biofilm formation through bacterial extracellular DNA release and platelet adhesion in experimental endocarditis | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1371/JOURNAL.PPAT.1009289 | - |
dc.identifier.pmid | 33577624 | - |
dc.identifier.scopus | 2-s2.0-85102394315 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85102394315 | - |
dc.relation.journalvolume | 17 | en_US |
dc.relation.journalissue | 2 | en_US |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Pathology-NTUCC | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUHHC | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Clinical Dentistry | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oral Biology | - |
crisitem.author.dept | Microbiology | - |
crisitem.author.dept | Dentistry-NTUH | - |
crisitem.author.dept | School of Dentistry | - |
crisitem.author.orcid | 0000-0001-5648-9202 | - |
crisitem.author.orcid | 0000-0003-1438-9187 | - |
crisitem.author.orcid | 0000-0002-5797-6093 | - |
crisitem.author.orcid | 0000-0002-7794-497X | - |
crisitem.author.orcid | 0000-0002-5431-0712 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | NTU Hsin-Chu Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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