https://scholars.lib.ntu.edu.tw/handle/123456789/566073
標題: | Chinese olive (Canarium album L.) fruit regulates glucose utilization by activating AMP-activated protein kinase | 作者: | Yeh, Y.-T. TING-JANG LU Lian, G.-T. Lung, M.-C. Lee, Y.-L. Chiang, A.-N. SHU-CHEN HSIEH |
關鍵字: | AMPK; Chinese olive (Canarium album L.); glucose uptake; high-fat diet; mitochondrial | 公開日期: | 2020 | 卷: | 34 | 期: | 6 | 起(迄)頁: | 7866-7884 | 來源出版物: | FASEB Journal | 摘要: | A growing body of evidence demonstrates obesity-induced insulin resistance is associated with the development of metabolic diseases. This study was designed to investigate ethyl acetate fraction of Chinese olive fruit extract (CO-EtOAc)-mediated attenuation of obesity and hyperglycemia in a mouse model. About 60% HFD-fed mice were treated intragastrically with CO-EtOAc for last 6?weeks, and body weight, blood biochemical parameters as well as hepatic inflammation response were investigated. Our results showed that CO-EtOAc treatment significantly reduced the formation of hepatic lipid droplets, body weight gain, blood glucose, and improved serum biochemical parameters in HFD-induced obese and insulin resistant mice. We further explored the molecular mechanism underlying the blood glucose modulating effect of CO-EtOAc using L6 myotubes model. We conclude that CO-EtOAc effectively increases the glycogen content and glucose uptake by stimulating the membrane translocation of glucose transporter 4. In addition, CO-EtOAc depolarizes the mitochondrial membrane and decreases the mitochondrial oxygen consumption, which may result in AMPK activation and the consequent mitochondrial fission. This study shows that CO-EtOAc prevents the development of obesity in mice fed with HFD and is also capable of stimulating glucose uptake. The possible mechanism might be due to the effects of CO-EtOAc on activation of AMPK and promotion of mitochondrial fission. ? 2020 Federation of American Societies for Experimental Biology |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85084069904&partnerID=40&md5=9fe188315885c0960fc9b6aff06d178b https://scholars.lib.ntu.edu.tw/handle/123456789/566073 |
DOI: | 10.1096/fj.201902494RR | SDG/關鍵字: | acetic acid ethyl ester; adenine nucleotide translocase; canarium album extract; caspase 3; cholesterol; ellagic acid; ethyl gallate; gallic acid; gallic acid derivative; gallic acid methyl ester; glucose transporter 4; glycogen synthase kinase 3beta; hydroxymethylglutaryl coenzyme A reductase kinase; lactic acid; natural product; plant extract; reduced nicotinamide adenine dinucleotide dehydrogenase; succinate dehydrogenase; triacylglycerol; unclassified drug; antidiabetic agent; glucose; glucose transporter; hydroxymethylglutaryl coenzyme A reductase kinase; insulin; plant extract; animal experiment; animal tissue; Article; body weight; body weight change; Canarium album; cell differentiation; cell fractionation; confocal microscopy; controlled study; drug mechanism; enzyme activation; fasting; fruit; glucose blood level; glucose homeostasis; glucose transport; glucose utilization; glycogen analysis; histology; hyperglycemia; in vitro study; in vivo study; lipid diet; lipid liver level; liquid chromatography-mass spectrometry; live cell imaging; liver tissue; male; mitochondrial membrane potential; mitochondrial respiration; mouse; mouse model; nonhuman; obesity; olive; oxygen consumption; priority journal; protein phosphorylation; skeletal muscle cell; Taiwan; Western blotting; adverse event; animal; C57BL mouse; chemistry; drug effect; experimental diabetes mellitus; fruit; insulin resistance; liver; metabolism; obesity; olive tree; phosphorylation; physiology; AMP-Activated Protein Kinases; Animals; Blood Glucose; Body Weight; Diabetes Mellitus, Experimental; Diet, High-Fat; Fruit; Glucose; Glucose Transport Proteins, Facilitative; Hypoglycemic Agents; Insulin; Insulin Resistance; Liver; Male; Mice; Mice, Inbred C57BL; Obesity; Olea; Phosphorylation; Plant Extracts; Taiwan |
顯示於: | 食品科技研究所 |
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