https://scholars.lib.ntu.edu.tw/handle/123456789/566723
標題: | Human immunodeficiency viruses regulated by alternative trans-activators: Genetic evidence for a novel non-transcriptional function of Tat in virion infectivity | 作者: | LI-MIN HUANG Joshi A. Willey R. Orenstein J. Jeang K.-T. |
關鍵字: | Cytopathicity; HIV; TAT; Transcription regulation; Virus infectivity | 公開日期: | 1994 | 出版社: | Wiley-VCH Verlag | 卷: | 13 | 期: | 12 | 起(迄)頁: | 2886-2896 | 來源出版物: | EMBO Journal | 摘要: | Thirteen genetically altered HIV-1 proviruses were created. These various genomes can be segregated into three groups: (i) a set of tat(-) viruses that have a functional HTLV-I Tax inserted in nef; (ii) a set of tat(-) viruses with Gal4 binding sites inserted in U3 and a Gal4-VP16 cDNA inserted in nef; and (iii) a set of tat(+) HIV genomes that are 5' and 3' TAR(-) and are Gal4-binding-site(+) in U3 and Gal4-VP16(+) in nef. We found that viruses in groups (i) and (ii), although tat(-), were fully complemented for viral gene expression based on quantitative measurements of viral protein synthesis and on the visualization by electron microscopy of the proper assembly of morphologically correct virions. Interestingly, group (i) and (ii) virions were defective in a spreading cytopathic infection when assayed in T-lymphocytes. Group (iii) viruses, although capable of producing intact Tat protein, also could not use Tat for transcription/gene expression because of the TAR(-) genotype. However, this class of viral genomes produced viruses that were highly infectious and cytopathic in primary and in continuously propagated T-lymphocytes. These three groups of viruses are all transcriptionally Tat-TAR independent. Their distinct differences in infectivity/cytopathicity provide genetic evidence that Tat provides a transcriptionally independent function in determining infectivity and cytopathicity in the setting of a spreading viral infection. Given that all HIV virions normally contain four intact copies of TAR RNA, our findings suggest a re-examination of whether Tat could be a virion-TAR-associated protein and the possible implications of this for virus infectivity/cytopathicity. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028318363&doi=10.1002%2fj.1460-2075.1994.tb06583.x&partnerID=40&md5=065f0c04147517e7916b084a84bf6e31 https://scholars.lib.ntu.edu.tw/handle/123456789/566723 |
ISSN: | 0261-4189 | DOI: | 10.1002/j.1460-2075.1994.tb06583.x | SDG/關鍵字: | Tax protein; transactivator protein; article; gene expression; Human immunodeficiency virus 1; nonhuman; priority journal; provirus; T lymphocyte; transcription regulation; virion; virus gene; virus infectivity; virus mutant; HTLV; Human immunodeficiency virus; Human immunodeficiency virus 1; Human T-lymphotropic virus 1 |
顯示於: | 醫學系 |
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