https://scholars.lib.ntu.edu.tw/handle/123456789/567591
標題: | Effects of fenofibrate on adiponectin expression in retinas of streptozotocin-induced diabetic rats | 作者: | Hsu Y.-J. Wang L.-C. WEI-SHIUNG YANG CHUNG-MAY YANG CHANG-HAO YANG |
公開日期: | 2014 | 出版社: | Hindawi Limited | 卷: | 2014 | 起(迄)頁: | 540326 | 來源出版物: | Journal of Diabetes Research | 摘要: | Adiponectin has been associated with increased risks of microvascular complications in diabetes; however, its role in the development of diabetic retinopathy (DR) is unknown. Fenofibrate is a lipid-lowering agent that has been shown to be capable of preventing DR progression. We investigated the expression of adiponectin and its receptors in DR and evaluated the effects of fenofibrate on their expression. The mRNA and protein levels of adiponectin and its receptors were elevated in retinas of streptozotocin-induced diabetic rats and were suppressed following fenofibrate treatment. Immunofluorescence staining demonstrated that adiponectin and adipoR1 were expressed in cells located within blood vessels, the retinal ganglion, and the inner nuclear layer. AdipoR1 was strongly expressed whereas adipoR2 was only weekly expressed in vascular endothelial cells.The in vitro experiments showed that adiponectin expression was induced by high glucose concentrations in RGC-5 and RAW264.7 cells and was suppressed following fenofibrate treatment. AdipoR1 and adipoR2 levels in RGC-5 cells were elevated in high glucose concentrations and suppressed by fenofibrate. Our results demonstrated that adiponectin may be a proinflammatory mediator in diabetic retinas and fenofibrate appears to modulate the expression of adiponectin and its receptors in diabetic retinas, effectively reducing DR progression. Copyright ? 2014 Ying-Jung Hsu et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84918509961&doi=10.1155%2f2014%2f540326&partnerID=40&md5=8cd27aee8da6e6b9e5013dfcbdc601ca https://scholars.lib.ntu.edu.tw/handle/123456789/567591 |
ISSN: | 2314-6745 | DOI: | 10.1155/2014/540326 | SDG/關鍵字: | adiponectin; adiponectin receptor 1; adiponectin receptor 2; cholesterol; fenofibrate; glucose; messenger RNA; streptozocin; adiponectin; adiponectin receptor; adiponectin receptor 1, rat; adiponectin receptor 2, rat; Adipoq protein, rat; antiinflammatory agent; autacoid; fenofibrate; glucose blood level; messenger RNA; animal cell; animal experiment; animal model; Article; blood vessel; cellular distribution; cholesterol blood level; controlled study; diabetic retinopathy; drug effect; endothelium cell; female; ganglion; glucose blood level; immunofluorescence; in vitro study; in vivo study; mouse; nonhuman; protein expression; rat; retina; retina nerve cell; vascular endothelium; animal; aqueous humor; blood; cell line; chemically induced; Diabetes Mellitus, Experimental; diabetic retinopathy; drug effects; genetics; metabolism; Sprague Dawley rat; Adiponectin; Animals; Anti-Inflammatory Agents; Aqueous Humor; Blood Glucose; Cell Line; Diabetes Mellitus, Experimental; Diabetic Retinopathy; Female; Fenofibrate; Inflammation Mediators; Mice; Rats, Sprague-Dawley; Receptors, Adiponectin; Retina; RNA, Messenger; Streptozocin |
顯示於: | 醫學系 |
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