|Title:||Administration of polysaccharides from Antrodia camphorata modulates dendritic cell function and alleviates allergen-induced T helper type 2 responses in a mouse model of asthma||Authors:||Liu K.-J.
|Keywords:||Antrodia camphorata; Asthma; Dendritic cells; T cells||Issue Date:||2010||Journal Volume:||129||Journal Issue:||3||Start page/Pages:||351-362||Source:||Immunology||Abstract:||
Summary Asthma is a chronic disease characterized by airway inflammation caused by the dysregulated production of cytokines secreted by allergen-specific type 2 T helper (Th2) cells. Antrodia camphorata is a commonly used fungus in Asian folk medicine, and A. camphorata polysaccharides are reported to possess anti-cancer activities. In this study, the immunomodulatory effects of purified fractionated polysaccharides (GF2) from A. camphorata on dendritic cells (DCs) and their potential preventive effects against ovalbumin (OVA) -induced asthma were investigated. In the presence of GF2, lipopolysaccharide (LPS) -activated DCs exhibited up-regulated expression of major histocompatibility complex (MHC) class II and co-stimulatory molecules, as well as enhanced interleukin-10 (IL-10) and IL-12 production. GF2 treatment on LPS-activated DCs suppressed na?ve CD4+ T-cell proliferation and Th2 cell polarization with IL-10 production in an allogeneic mixed lymphocyte reaction. In animal experiments, a high dose of GF2 efficiently reduced expression levels of OVA-specific immunoglobulin G1 (IgG1) and IgE. However, lower doses of GF2 significantly enhanced OVA-specific IgG2a production. Our data also showed that administration of GF2 dose-dependently inhibited the development of airway hyperresponsiveness, airway eosinophilia and Th2 responses. OVA-specific CD4+ T cells from higher doses of GF2-treated mice had significantly lower proliferative capacities compared with control mice. Moreover, treatment with GF2 significantly increased the high levels of IL-10 and low levels of interferon-γ produced by T cells. Taken together, these data indicate that administration of A. camphorata polysaccharides (GF2) may have therapeutic potential when used as an adjuvant for the immunomodulatory treatment of allergic asthma. ? 2009 Blackwell Publishing Ltd.
|ISSN:||0019-2805||DOI:||10.1111/j.1365-2567.2009.03175.x||SDG/Keyword:||allergen; antiasthmatic agent; gamma interferon; immunoglobulin E; immunoglobulin G1; immunoglobulin G2a; interleukin 10; interleukin 12; interleukin 13; interleukin 4; interleukin 5; lipopolysaccharide; major histocompatibility antigen class 2; ovalbumin; polysaccharide; polysaccharide gf2; transforming growth factor beta; unclassified drug; allergic asthma; animal experiment; animal model; Antrodia camphorata; article; CD4+ T lymphocyte; cell function; cell polarity; cell proliferation; control group; controlled study; cytokine production; dendritic cell; enzyme linked immunosorbent assay; experimental model; experimental mouse; female; flow cytometry; fluorescence activated cell sorting; fungus; immune response; immunization; medicinal plant; mixed lymphocyte reaction; mouse; mushroom; nonhuman; priority journal; reverse transcription polymerase chain reaction; Th2 cell; upregulation; Allergens; Animals; Antrodia; Asthma; Bronchial Hyperreactivity; Bronchial Provocation Tests; Bronchoalveolar Lavage Fluid; Cell Differentiation; Cell Proliferation; Cell Survival; Cytokines; Dendritic Cells; Female; Gene Expression; Immunoglobulin E; Immunoglobulin G; Interleukin-10; Interleukin-12; Lipopolysaccharides; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Ovalbumin; Polysaccharides; Pulmonary Eosinophilia; Spleen; T-Lymphocytes; Th2 Cells; Vaccination
|Appears in Collections:||醫學系|
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