|Title:||Defective functions of circulating CD4+CD25+ and CD4+CD25- T cells in patients with chronic ordinary urticaria||Authors:||Chen W.-C.
|Keywords:||CD4+CD25+ regulatory T cells; CD4+CD25- T cells; Chronic ordinary urticaria; Cytokines; FOXP3; Suppressive function||Issue Date:||2008||Journal Volume:||51||Journal Issue:||2||Start page/Pages:||121-130||Source:||Journal of Dermatological Science||Abstract:||
Background: Patients with chronic ordinary urticaria (CU) are divided into two groups: 30-50% have chronic autoimmune urticaria, and the remainder have chronic idiopathic urticaria. CD4+CD25+ regulatory T (Treg) cells play critical roles in maintaining peripheral tolerance and preventing autoimmunity, but the characteristics of Treg cells have not yet been defined in CU. Objective: To identify whether CD4+ T cells play an important immunoregulatory role in the etiology of CU, we determined the frequencies and functions of circulating CD4+CD25+ and CD4+CD25- T cells in CU patients and healthy control subjects, with special focus on the characteristics of CD4+CD25+ T cells. Methods: Peripheral blood mononuclear cells (PBMCs) were obtained from CU and healthy controls in this study. The frequency of CD4+CD25+ T cells in PBMCs was detected by flow cytometry. The expression levels of forkhead box P3 (FOXP3) and transforming growth factor-β (TGF-β) in CD4+CD25+ T cells were detected by real-time PCR. Furthermore, the suppressive function of CD4+CD25+ T cells was analyzed. Additionally, the Th1/Th2 cytokine secretory profile in mitogen-stimulated CD4+CD25- T cells was measured by ELISA. Results: An increased frequency of CD4+CD25+ T cells was observed in CU patients (n = 19) compared to control subjects (n = 7). No significant difference was detected in the expression levels of FOXP3 or TGF-β between CU patients (n = 14) and control subjects (n = 7). Strikingly, the suppressive capacity of CD4+CD25+ Treg cells from 2 of 5 CU patients was partially defective. We also found that cytokine production from CD4+CD25- T cells was significantly reduced in CU patients (n = 9) compared to healthy donors (n = 11). Conclusions: Our data demonstrate that CD4+CD25+ and CD4+CD25- T cells in PBMCs exhibit defective functions in CU patients. ? 2008 Japanese Society for Investigative Dermatology.
|ISSN:||0923-1811||DOI:||10.1016/j.jdermsci.2008.02.012||SDG/Keyword:||mitogenic agent; transcription factor FOXP3; transforming growth factor beta; adolescent; adult; article; autoimmune disease; autoimmunity; CD4+ CD25+ T lymphocyte; CD4+ T lymphocyte; child; chronic urticaria; clinical article; controlled study; cytokine production; cytokine release; enzyme linked immunosorbent assay; female; flow cytometry; human; human cell; immunological tolerance; immunoregulation; lymphocyte function; lymphocyte proliferation; male; peripheral blood mononuclear cell; priority journal; protein expression; real time polymerase chain reaction; regulatory T lymphocyte; Th1 cell; Th2 cell; Adolescent; Adult; Antigens, CD3; Autoimmunity; Case-Control Studies; Cell Proliferation; Child; Child, Preschool; Chronic Disease; Female; Forkhead Transcription Factors; Humans; Interleukin-2 Receptor alpha Subunit; Leukocytes, Mononuclear; Male; RNA, Messenger; T-Lymphocytes, Regulatory; Transforming Growth Factor beta; Urticaria
|Appears in Collections:||醫學系|
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