https://scholars.lib.ntu.edu.tw/handle/123456789/568164
標題: | Intellectual disability-associated dBRWD3 regulates gene expression through inhibition of HIRA/YEM-mediated chromatin deposition of histone H3.3 | 作者: | Chen W.-Y. Shih H.-T. Liu K.-Y. Shih Z.-S. Chen L.-K. Tsai T.-H. Chen M.-J. Liu H. Tan B.C.-M. CHIEN-YU CHEN HSIU-HSIANG LEE Loppin B. Aït‐Ahmed O. Wu J.-T. |
關鍵字: | BRWD3; HIRA; histone H3.3; intellectual disability; YEM | 公開日期: | 2015 | 出版社: | Wiley-VCH Verlag | 卷: | 16 | 期: | 4 | 起(迄)頁: | 528-538 | 來源出版物: | EMBO Reports | 摘要: | Many causal mutations of intellectual disability have been found in genes involved in epigenetic regulations. Replication-independent deposition of the histone H3.3 variant by the HIRA complex is a prominent nucleosome replacement mechanism affecting gene transcription, especially in postmitotic neurons. However, how HIRA-mediated H3.3 deposition is regulated in these cells remains unclear. Here, we report that dBRWD3, the Drosophila ortholog of the intellectual disability gene BRWD3, regulates gene expression through H3.3, HIRA, and its associated chaperone Yemanuclein (YEM), the fly ortholog of mammalian Ubinuclein1. In dBRWD3 mutants, increased H3.3 levels disrupt gene expression, dendritic morphogenesis, and sensory organ differentiation. Inactivation of yem or H3.3 remarkably suppresses the global transcriptome changes and various developmental defects caused by dBRWD3 mutations. Our work thus establishes a previously unknown negative regulation of H3.3 and advances our understanding of BRWD3-dependent intellectual disability. Synopsis BRWD3 is mutated in patients with X-linked intellectual disability. Here, dBRWD3 is found to negatively regulate the amount of chromatin-associated H3.3, thereby regulating gene expression in the brain and several developmental processes. dBRWD3 mutation causes various developmental abnormalities by altering gene expression. HIRA/YEM-mediated H3.3 deposition is increased in dBRWD3 mutants. The transcriptomic changes and diverse developmental defects of dBRWD3 mutants are suppressed by inactivation of the HIRA/YEM-H3.3 pathway. BRWD3 is mutated in patients with X-linked intellectual disability. Here, dBRWD3 is found to negatively regulate the amount of chromatin-associated H3.3, thereby regulating gene expression in the brain and several developmental processes. ? 2015 The Authors. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84926254159&doi=10.15252%2fembr.201439092&partnerID=40&md5=120a781efe79485523afec754bfa6c5e https://scholars.lib.ntu.edu.tw/handle/123456789/568164 |
ISSN: | 1469-221X | DOI: | 10.15252/embr.201439092 |
顯示於: | 分子醫學研究所 |
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