https://scholars.lib.ntu.edu.tw/handle/123456789/568454
標題: | Polymerase chain reaction with phase change as intrinsic thermal control | 作者: | Hsieh, Y.-F. Yonezawa, E. Kuo, L.-S. Shiou-Hwei Yeh PEI-JER CHEN PING-HEI CHEN |
公開日期: | 2013 | 出版社: | American Institute of Physics Inc. | 卷: | 102 | 期: | 17 | 起(迄)頁: | 173701 | 來源出版物: | Applied Physics Letters | 摘要: | This research demonstrated that without any external temperature controller, the capillary convective polymerase chain reaction (ccPCR) powered by a candle can operate with the help of phase change. The candle ccPCR system productively amplified hepatitis B virus 122 base-pairs DNA fragment. The detection sensitivity can achieve at an initial DNA concentration to 5 copies per reaction. The results also show that the candle ccPCR system can operate functionally even the ambient temperature varies from 7 °C to 45 °C. These features imply that the candle ccPCR system can provide robust medical detection services. ? 2013 AIP Publishing LLC. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983718347&doi=10.1063%2f1.4803442&partnerID=40&md5=73b9ebe9de5361efc809b92ebafad74a https://scholars.lib.ntu.edu.tw/handle/123456789/568454 |
ISSN: | 0003-6951 | DOI: | 10.1063/1.4803442 | SDG/關鍵字: | Viruses; Detection sensitivity; DNA concentration; DNA fragment; External temperature; Hepatitis B virus; Phase Change; Thermal control; Polymerase chain reaction |
顯示於: | 臨床醫學研究所 |
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