https://scholars.lib.ntu.edu.tw/handle/123456789/568511
標題: | Fine mapping of hepatitis B virus pre-S deletion and its association with hepatocellular carcinoma | 作者: | JIA-HORNG KAO CHUN-JEN LIU Jow G.-M. PEI-JER CHEN DING-SHINN CHEN Chen B.-F. |
公開日期: | 2012 | 出版社: | Blackwell Publishing Ltd | 卷: | 32 | 期: | 9 | 起(迄)頁: | 1373-1381 | 來源出版物: | Liver International | 摘要: | Background: Naturally occurring pre-S deletion mutants have been identified in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Aims: This study investigated whether specific deletions within the pre-S region were associated with HCC development. Methods: The virologic characteristics of 56 HBV chronic carriers and 112 age-matched patients with HBV-related HCC were examined. Results: The HCC patients had a significantly higher frequency of high viral load, basal core promoter mutation and pre-S deletion than chronic carriers. Sequencing analysis showed that the deleted regions were clustered mainly in the C terminus of pre-S1 (70.5%) and the N terminus of pre-S2 (72.7%) in HCC patients. Immuno-epitope mapping of these pre-S deletion sequences showed that all the deletion regions encompassed T- and B- cell epitopes and the B-cell epitope at amino acid 1-6 of pre-S2 was significantly deleted in HCC patients (60.0% vs. 0.0%; P = 0.036). Functional mapping of these deletion mutants showed that most of HCC patients lost one or more functional sites and the deletion of site for viral secretion (aa 1-5 of pre-S2 domain) was significantly detected in HCC patients than chronic carriers (62.5% vs. 0.0%; P = 0.029). Computational protein function prediction indicated that these mutants may have different molecular functions and participate in other biological processes compared with wild-type pre-S. Conclusions: Deletion of B-cell epitope at amino acid 1-6 of pre-S2 region and the site for virion secretion are significantly associated with the development of HCC in HBV carriers. ? 2012 John Wiley & Sons A/S. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984535405&doi=10.1111%2fj.1478-3231.2012.02826.x&partnerID=40&md5=4db22e2bb59166945ddc18cc05226df6 https://scholars.lib.ntu.edu.tw/handle/123456789/568511 |
ISSN: | 1478-3223 | DOI: | 10.1111/j.1478-3231.2012.02826.x | SDG/關鍵字: | epitope; adult; amino terminal sequence; article; B lymphocyte; cancer patient; carboxy terminal sequence; deletion mutant; epitope mapping; female; gene mutation; gene sequence; hepatitis B; Hepatitis B virus; human; liver cell carcinoma; major clinical study; male; nonhuman; pre s deletion mutant; T lymphocyte; virus load; wild type |
顯示於: | 臨床醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。