https://scholars.lib.ntu.edu.tw/handle/123456789/568605
標題: | A micro circulating PCR chip using a suction-type membrane for fluidic transport | 作者: | Chien L.-J. Wang J.-H. Hsieh T.-M. Chen P.-H. PEI-JER CHEN Lee D.-S. Luo C.-H. Lee G.-B. |
關鍵字: | MEMS; Microfluidics; Microheaters; Molecular diagnosis; PCR | 公開日期: | 2009 | 出版社: | Springer New York LLC | 卷: | 11 | 期: | 2 | 起(迄)頁: | 359-367 | 來源出版物: | Biomedical Microdevices | 摘要: | A new micromachined circulating polymerase chain reaction (PCR) chip is reported in this study. A novel liquid transportation mechanism utilizing a suction-type membrane and three microvalves were used to create a new microfluidic control module to rapidly transport the DNA samples and PCR reagents around three bio-reactors operating at three different temperatures. When operating at a membrane actuation frequency of 14.29 Hz and a pressure of 5 psi, the sample flow rate in the microfluidic control module can be as high as 18 μL/s. In addition, an array-type microheater was adopted to improve the temperature uniformity in the reaction chambers. Open-type reaction chambers were designed to facilitate temperature calibration. Experimental data from infrared images showed that the percentage of area inside the reaction chamber with a thermal variation of less than 1°C was over 90% for a denaturing temperature of 94°C. Three array-type heaters and temperature sensors were integrated into this new circulating PCR chip to modulate three specific operating temperatures for the denaturing, annealing, and extension steps of a PCR process. With this approach, the cycle numbers and reaction times of the three separate reaction steps can be individually adjusted. To verify the performance of this circulating PCR chip, a PCR process to amplify a detection gene (150 base pairs) associated with the hepatitis C virus was performed. Experimental results showed that DNA samples with concentrations ranging from 105 to 102copies/μL can be successfully amplified. Therefore, this new circulating PCR chip may provide a useful platform for genetic identification and molecular diagnosis. ? Springer Science+Business Media, LLC 2008. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-62649124715&doi=10.1007%2fs10544-008-9242-z&partnerID=40&md5=f14a08b43abc3385a98500b59d8169de https://scholars.lib.ntu.edu.tw/handle/123456789/568605 |
ISSN: | 1387-2176 | DOI: | 10.1007/s10544-008-9242-z | SDG/關鍵字: | Bioassay; Computer viruses; Cosmic rays; Diagnosis; DNA; Genes; Heating equipment; Infrared imaging; MEMS; Microelectromechanical devices; Microfluidics; Molecular biology; Nucleic acids; Pumps; A thermals; Array types; Array-type heaters; Base pairs; Cycle numbers; DNA samples; Experimental datum; Genetic identifications; Hepatitis C virus; Infrared images; Liquid transportations; Membrane actuations; Micro-valves; Microfluidic controls; Microheaters; Micromachined; Molecular diagnosis; Operating temperatures; PCR; PCR chips; Polymerase chain reactions; Reaction chambers; Reaction steps; Reaction time; Temperature calibrations; Temperature uniformities; Biochips; article; bioreactor; DNA transfer; flow rate; fluid transport; gene amplification; Hepatitis C virus; membrane transport; microchip analysis; microfluidics; polymerase chain reaction; priority journal; thermoregulation; Hepatitis C virus; Chemical Fractionation; Equipment Design; Equipment Failure Analysis; Flow Injection Analysis; Heating; Membranes, Artificial; Micro-Electrical-Mechanical Systems; Microfluidic Analytical Techniques; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; RNA; Sensitivity and Specificity; Suction |
顯示於: | 臨床醫學研究所 |
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