https://scholars.lib.ntu.edu.tw/handle/123456789/568708
標題: | Intratypic recombination among lineages of type 1 vaccine-derived poliovirus emerging during chronic infection of an immunodeficient patient | 作者: | Yang C.-F. Chen H.-Y. Jorba J. Sun H.-C. Yang S.-J. Lee H.-C. Huang Y.-C. Lin T.-Y. PEI-JER CHEN Shimizu H. Nishimura Y. Utama A. Pallansch M. Miyamura T. Kew O. Yang J.-Y. |
公開日期: | 2005 | 出版社: | American Society for Microbiology | 卷: | 79 | 期: | 20 | 起(迄)頁: | 12623-12634 | 來源出版物: | Journal of Virology | 摘要: | We determined the complete genomic sequences of nine type 1 immunodeficient vaccine-derived poliovirus (iVDPV) isolates obtained over a 337-day period from a poliomyelitis patient from Taiwan with common variable immunodeficiency. The iVDPV isolates differed from the Sabin type 1 oral poliovirus vaccine (OPV) strain at 1.84% to 3.15% of total open reading frame positions and had diverged into at least five distinct lineages. Phylogenetic analysis suggested that the chronic infection was initiated by the fifth and last OPV dose, given 567 days before onset of paralysis, and that divergence of major lineages began very early in the chronic infection. Key determinants of attenuation in Sabin 1 had reverted in the iVDPV isolates, and representative isolates of each lineage showed increased neurovirulence for PVR-Tg21 transgenic mice. None of the isolates had retained the temperature-sensitive phenotype of Sabin 1. All isolates were antigenic variants of Sabin 1, having multiple amino acid substitutions within or near neutralizing antigenic sites 1, 2, and 3a. Antigenic divergence of the iVDPV variants from Sabin 1 followed two major independent evolutionary pathways. The emergence of distinct coreplicating lineages suggests that iVDPVs can replicate for many months at separate sites in the gastrointestinal tract. Some isolates had mosaic genome structures indicative of recombination across and within lineages. iVDPV excretion apparently ceased after 30 to 35 months of chronic infection. The appearance of a chronic VDPV excretor in a tropical, developing country has important implications for the strategy to stop OPV immunization after eradication of wild polioviruses. Copyright ? 2005, American Society for Microbiology. All Rights Reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983719085&doi=10.1128%2fJVI.79.20.12623-12634.2005&partnerID=40&md5=c55d4404a1a108abe1afa1b4abee8cab https://scholars.lib.ntu.edu.tw/handle/123456789/568708 |
ISSN: | 0022-538X | DOI: | 10.1128/JVI.79.20.12623-12634.2005 | SDG/關鍵字: | oral poliomyelitis vaccine; amino acid substitution; animal experiment; article; case report; common variable immunodeficiency; developing country; female; gene sequence; genetic variability; human; immunization; male; mouse; nonhuman; nucleotide sequence; open reading frame; paralysis; phylogeny; poliomyelitis; Poliomyelitis virus; priority journal; school child; sequence analysis; Taiwan; transgenic mouse; tropics; type 1 immunodeficient vaccine derived poliovirus; virus excretion; virus genome; virus isolation; virus recombinant; virus recombination; virus replication; virus strain; virus virulence; Mus musculus; Poliovirus |
顯示於: | 臨床醫學研究所 |
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