https://scholars.lib.ntu.edu.tw/handle/123456789/568722
標題: | Diverse cellular transformation capability of overexpressed genes in human hepatocellular carcinoma | 作者: | Huang J.-S. Chao C.-C. Su T.-L. Shiou-Hwei Yeh DING-SHINN CHEN Chen C.-T. PEI-JER CHEN Jou Y.-S. |
關鍵字: | Cellular transformation; Hepatocellular carcinoma; Overexpression; Tong/HCC | 公開日期: | 2004 | 出版社: | Academic Press Inc. | 卷: | 315 | 期: | 4 | 起(迄)頁: | 950-958 | 來源出版物: | Biochemical and Biophysical Research Communications | 摘要: | For isolation of novel cellular transforming genes that potentially participated in hepatocarcinogenesis, we conducted anchorage-independent growth (AIG) assays on 10 human liver cancer cell lines and observed strong AIG capabilities in PLC5 and Huh7 but negligible in Tong cells. After cloning of genes by differential subtractive chain reactions (DSC) from strong AIG to AIG negative cells, we sequenced 2304 clones and identified 245 genes. After four stringent criteria for selection of transforming genes among DSC clones, our results of quantitative RT-PCR analysis indicated that six genes, DDX3, EIF3S2, CLIC1, HDGF, GPC3, and HSPCA were overexpressed in 64%, 62%, 60%, 58%, 49%, and 47%, respectively, of 45 hepatocellular carcinoma (HCC) tissues. The results of cellular transformation capability by AIG assays indicated that the transfectants of EIF3S2 showed the strongest (100-fold), DDX3 and CLIC1 were moderate, GPC3 and HSPCA were weak, and HDGF was none in forming colonies in soft agar. Together, our results suggested that Tong is a suitable human cell line for screening of overexpressed and/or cellular transforming genes. In addition, our results suggested that diverse functions of cellular transforming genes in various biological pathways could transform human Tong cells and potentially reveal new targets for drug development of HCC. ? 2004 Elsevier Inc. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983723471&doi=10.1016%2fj.bbrc.2004.01.151&partnerID=40&md5=8170f4b09dfc61560119e14f4ebe0536 https://scholars.lib.ntu.edu.tw/handle/123456789/568722 |
ISSN: | 0006-291X | DOI: | 10.1016/j.bbrc.2004.01.151 | SDG/關鍵字: | anchorage independent growth; article; cell transformation; clic1 gene; colony formation; controlled study; ddx3 gene; eif3s2 gene; gene identification; gene overexpression; gene sequence; gpc3 gene; hdgf gene; hspca gene; human; human cell; human tissue; liver cell carcinoma; molecular cloning; oncogene; priority journal |
顯示於: | 臨床醫學研究所 |
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