https://scholars.lib.ntu.edu.tw/handle/123456789/568731
Title: | A new thermal cycling mechanism for effective polymerase chain reaction in microliter volumes | Authors: | Lee D.S. Tsai C.Y. Yuan W.H. PEI-JER CHEN Chen P.H. |
Issue Date: | 2004 | Publisher: | Springer Verlag | Journal Volume: | 10 | Journal Issue: | 8-9 | Start page/Pages: | 579-584 | Source: | Microsystem Technologies | Abstract: | This study presents a new thermal cycler using infrared (IR) heating and water impingement cooling for polymerase chain reaction (PCR) amplification of 10 μl samples in thin glass capillary tubes. The thermal cycling system can achieve a temperature ramping-up rate of 65°C/s and a ramping-down rate of 80°C/s. Two other cooling mechanisms, natural convection and forced air convection, can also be used in the present system to obtain a ramping-down rate of 2°C/s and 6°C/s, respectively. The amplification of the 439 fragment of hepatitis B virus (HBV) DNA was successful. The PCR amplified products were analyzed by agarose gel electrophoresis with ethidium bromide staining for visualization. A comparison of results of the amplification products at three different ramping-down rates was made and the rapid thermal cycling of the present system can run DNA required amplification in 29 min for 20 thermal cycles that is only 1/3 the time spent in the conventional PCR machine used in comparison. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984576533&doi=10.1007%2fs00542-003-0334-7&partnerID=40&md5=a9f93c5ea1b2c23bd101c2122c9c1d92 https://scholars.lib.ntu.edu.tw/handle/123456789/568731 |
ISSN: | 0946-7076 | DOI: | 10.1007/s00542-003-0334-7 | SDG/Keyword: | Amplification; Cooling; Enzymes; Infrared heating; Natural convection; Thermal cycling; Visualization; Water; Convetional thermal cycler; Microcentrifuge tubes; Polymerase chain reactions (PCR); DNA |
Appears in Collections: | 臨床醫學研究所 |
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