https://scholars.lib.ntu.edu.tw/handle/123456789/568762
標題: | A prospective study characterizing full-length hepatitis B virus genomes during acute exacerbation | 作者: | CHUN-JEN LIU PEI-JER CHEN Lai M.-Y. JIA-HORNG KAO Chang C.-F. Wu H.-L. Shau W.-Y. DING-SHINN CHEN |
公開日期: | 2003 | 出版社: | W.B. Saunders | 卷: | 124 | 期: | 1 | 起(迄)頁: | 80-90 | 來源出版物: | Gastroenterology | 摘要: | Background & Aims: Hepatitis B virus (HBV) evolves rapidly in patients with chronic hepatitis B, and HBV variation may trigger acute exacerbation. To study this relationship, we investigated full-length viral sequences before, during, and after exacerbation. Methods: We prospectively studied 14 patients with exacerbation of hepatitis B, either spontaneously (n = 4) or after receiving various medical interventions (n = 10), and measured their serum alanine aminotransferase (ALT) and HBV DNA levels monthly. Full-length HBV genomes at baseline, at the peak of serum viral load, at ALT peak, and after ALT peak were obtained by polymerase chain reaction, sequenced, and compared. Replication activities of serial HBV variants were assayed by in vitro transfection. Results: Serum viral load was increased in all exacerbations. Viral peak preceded ALT peak in 13 (93%) of the 14 patients. At virologic peak, 12 patients (86%) harbored viral genome identical to the corresponding baseline genome. At and after ALT peak, 9 (64%) and 7 (50%) of the viral genomes remained identical to baseline, respectively. Mean nucleotide change per genome was 0.2 at virologic peak but increased to 4.4 and 8.1 at and after ALT peak, respectively. The replication potential of the viral variant that emerged during or after exacerbation was equivalent to that at baseline. Conclusions: Most exacerbations were preceded by an upsurge of serum HBV identical to the preexisting HBV strain. After exacerbation, about half of the patients were repopulated by a different viral variant, which was likely a result of immune selection. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984585828&doi=10.1053%2fgast.2003.50003&partnerID=40&md5=ffc6207f32efacabc422a99cddfb1dad https://scholars.lib.ntu.edu.tw/handle/123456789/568762 |
ISSN: | 0016-5085 | DOI: | 10.1053/gast.2003.50003 | SDG/關鍵字: | nucleotide; acute hepatitis; adult; aged; alanine aminotransferase blood level; article; clinical article; controlled study; disease exacerbation; DNA virus; enzyme assay; female; genetic transfection; Hepatitis B virus; human; in vitro study; male; polymerase chain reaction; priority journal; prospective study; virus activation; virus genome; virus load; virus replication |
顯示於: | 臨床醫學研究所 |
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