https://scholars.lib.ntu.edu.tw/handle/123456789/568765
標題: | Characterization of integration patterns and flanking cellular sequences of hepatitis B virus in childhood hepatocellular carcinomas | 作者: | Tsuei D.-J. MEI-HWEI CHANG PEI-JER CHEN Hsu T.-Y. YEN-HSUAN NI |
公開日期: | 2002 | 出版社: | Wiley-Liss Inc. | 卷: | 68 | 期: | 4 | 起(迄)頁: | 513-521 | 來源出版物: | Journal of Medical Virology | 摘要: | Hepatitis B virus (HBV) DNA integration into host chromosomes is detected in more than 80% of HBV-related hepatocellular carcinomas (HCC), yet its significance in tumor development remains obscure. In this study, we re-examined the integration pattern of HBV in childhood HCC tissues, which has less environmental confounding factors than adult HCC. The HBV junctions and flanking cellular sequences were amplified from five childhood HCC patients by the inverse polymerase chain reaction (IPCR) method using primers located near HBV direct repeats (DR) 1 and 2. The viral junctions in nine of the ten obtained IPCR clones were demonstrated to be located near HBV DR1, and their patterns were classified to type I integrants. Southern blot analyses demonstrate that the cellular junctions derived from two of the five HCC tissues were male specific and contained sequences homologous to human long interspersed DNA elements (LINE-1). HBV integrant of one HCC tissue (1217T) was integrated into a RNA binding motif Y chromosome (RBMY) gene. The expression of RBMY, which is normally found only in male germ cells, was detected in HCC tissue 1217T by RT-PCR but not in the corresponding non-tumor liver tissue. The prevalence of RBMY expression in liver tissues from the tumor and non-tumor parts often other HCC children and seven biliary atresia (BA) children was studied by RT-PCR. No RBMY transcripts were detected in the non-tumor parts of HCC patients or the cirrhotic livers of BA children, whereas 30% (three of ten) of HCC tissues specifically expressed RBMY. The results indicate that HBV integration and activation of RBMY gene expression in liver cells may be associated with the development of childhood HCC. ? 2002 Wiley-Liss, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984537206&doi=10.1002%2fjmv.10240&partnerID=40&md5=86f6bfa76e573abe6d9d904834664e79 https://scholars.lib.ntu.edu.tw/handle/123456789/568765 |
ISSN: | 0146-6615 | DOI: | 10.1002/jmv.10240 | SDG/關鍵字: | gene product; RNA binding protein; virus DNA; adolescent; article; bile duct atresia; child; clinical article; clone; controlled study; DNA flanking region; gene expression; gene sequence; germ cell; hepatitis B; Hepatitis B virus; host parasite interaction; human; human tissue; inverse polymerase chain reaction; liver cell carcinoma; liver cirrhosis; long interspersed repeat; male; nonhuman; nucleotide sequence; protein motif; reverse transcription polymerase chain reaction; RNA binding; sequence analysis; sequence homology; Southern blotting; virus DNA cell DNA interaction; Y chromosome; Hepatitis B virus |
顯示於: | 臨床醫學研究所 |
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