https://scholars.lib.ntu.edu.tw/handle/123456789/568769
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | LI-HUI TSENG | en_US |
dc.contributor.author | PEI-JER CHEN | en_US |
dc.contributor.author | Lin M.-T. | en_US |
dc.contributor.author | Singleton K. | en_US |
dc.contributor.author | Martin E.G. | en_US |
dc.contributor.author | Yen A.-H. | en_US |
dc.contributor.author | Martin P.J. | en_US |
dc.contributor.author | Hansen J.A. | en_US |
dc.date.accessioned | 2021-07-03T03:35:44Z | - |
dc.date.available | 2021-07-03T03:35:44Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0022-1759 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983723321&doi=10.1016%2fS0022-1759%2802%2900165-5&partnerID=40&md5=4917696f453b8f5d74a92495a7a436d1 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/568769 | - |
dc.description.abstract | The interleukin-1 (IL-1) gene complex consists of the IL-1α, IL-1β and IL-1 receptor antagonist genes. Single-nucleotide polymorphisms (SNP) in all three genes have been associated with human diseases. In this study, primers containing mismatches at 1-3 nucleotide positions were designed to incorporate a restriction site for endonuclease AlwNI or XcmI in the presence of allele-specific nucleotides at the polymorphic positions. Based on this technique, a simple and robust multiplex polymerase chain reaction/restriction fragment length polymorphism (multiplex PCR/RFLP) assay was developed to determine simultaneously three to four informative SNPs (IL-1β/+3954, IL-1β/-511 and IL-1Ra/9261 or IL-1α/-889, IL-1β/-31, IL-1β/5810 and IL-1Ra/11100 SNPs) in the IL-1 gene complex. ? 2002 Elsevier Science B.V. All rights reserved. | - |
dc.publisher | Elsevier | - |
dc.relation.ispartof | Journal of Immunological Methods | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | endonuclease; interleukin 1 complex; interleukin 1 receptor blocking agent; interleukin 1alpha; interleukin 1beta; nucleotide; unclassified drug; allele; article; comparative study; genetic analysis; genotype; nucleotide sequence; polymerase chain reaction; priority journal; restriction fragment length polymorphism; restriction mapping; restriction site; single nucleotide polymorphism | - |
dc.title | Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/S0022-1759(02)00165-5 | - |
dc.identifier.scopus | 2-s2.0-84983723321 | - |
dc.relation.pages | 151-156 | - |
dc.relation.journalvolume | 267 | - |
dc.relation.journalissue | 2 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.orcid | 0000-0002-8308-6772 | - |
crisitem.author.orcid | 0000-0001-8316-3785 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 臨床醫學研究所 |
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