https://scholars.lib.ntu.edu.tw/handle/123456789/568782
標題: | Sequence analysis of pre-S/surface and pre-core/core promoter genes of hepatitis B virus in chronic hepatitis C patients with occult HBV infection | 作者: | JIA-HORNG KAO PEI-JER CHEN Lai M.-Y. DING-SHINN CHEN |
公開日期: | 2002 | 出版社: | Wiley-Liss Inc. | 卷: | 68 | 期: | 2 | 起(迄)頁: | 216-220 | 來源出版物: | Journal of Medical Virology | 摘要: | Although occult hepatitis B virus (HBV) infection in individuals without detectable hepatitis B surface antigen (HBsAg) may occur and has been reported to be common in patients with chronic hepatitis C, the related molecular mechanisms remain unknown. With the polymerase chain reaction, serum HBV DNA was sought in 100 HBsAg-negative patients with chronic hepatitis C virus (HCV)-infection. In those with occult HBV infection, possible genomic variability of HBV was evaluated by amplification and direct sequencing of pre-S, surface, and pre-core/core promoter genes. In total, 10 of the 100 patients (10%) had detectable serum HBV DNA, documenting an occult HBV infection. A deletion mutant in the pre-S gene was found in one patient and mutations of the a determinant of HBsAg were observed in 2. In addition, a novel core promoter mutant (a dinucleotide substitution: T-to-C at nucleotide 1,802 and T-to-G at nucleotide 1,803, T1802C/T1803G) was found frequently in patients with occult HBV infection as compared to sex- and age-matched HBsAg-positive patients (80 vs. 10%, P < 0.001). In conclusion, the data suggest occult HBV infection is not uncommon in chronic hepatitis C patients in Taiwan, and a novel core promoter mutant may be associated with the absence of circulating HBsAg in these patients. ? 2002 Wiley-Liss, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984539092&doi=10.1002%2fjmv.10188&partnerID=40&md5=f9d90996ec57159dcb7573bd32bc9e5c https://scholars.lib.ntu.edu.tw/handle/123456789/568782 |
ISSN: | 0146-6615 | DOI: | 10.1002/jmv.10188 | SDG/關鍵字: | core protein; hepatitis B core antigen; hepatitis B surface antigen; virus DNA; adult; age; article; chronic hepatitis; deletion mutant; female; gender; gene amplification; gene deletion; genetic variability; hepatitis; Hepatitis B virus; hepatitis C; human; major clinical study; male; molecular mechanics; polymerase chain reaction; promoter region; sequence analysis; Taiwan; Hepatitis B virus; Hepatitis C virus |
顯示於: | 臨床醫學研究所 |
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