https://scholars.lib.ntu.edu.tw/handle/123456789/568839
標題: | Amplification of GB virus-C/hepatitis G virus RNA with primers from different regions of the viral genome | 作者: | JIA-HORNG KAO PEI-JER CHEN Chen W. Hsiang S.C. Lai M.Y. DING-SHINN CHEN |
公開日期: | 1997 | 卷: | 51 | 期: | 4 | 起(迄)頁: | 284-289 | 來源出版物: | Journal of Medical Virology | 摘要: | GB virus-C/hepatitis G virus (GBV-C/HGV) is a newly identified RNA virus. The aim of the study was to compare three primer pairs from the 5' untranslated region (5'UTR), envelope region 2 (E 2) and nonstructural region 3 (NS 3) of GBV-C/HGV genome for their ability to detect GBV-C/HGV RNA by polymerase chain reaction (PCR) assays. By using PCR with primers from different regions of the viral genome, serum GBV-C/HGV RNA was assayed in 200 at-risk individuals. The sensitivity of this assay was assessed by a titration experiment, and nucleotide sequences of the amplified products were determined directly. Of 200 serum samples, 43 (21.5%) were positive for GBV-C/HGV RNA with at least one of the primer pairs. The positive rates by 5'UTR, NS 3, and E 2 primers were 100%, 98%, and 84%, respectively, and the sensitivity of PCR assays using 5'UTR primers was 10 to 100 times more likely to detect GBV-C/HGV RNA than that of NS3 and E 2 primers. The average homology of amplified targets to the prototype HGV genome was 89%, 80%, and 85% and the similarity between each amplified target was up to 100%, 90%, and 92% in the 5'UTR, E 2, and NS 3 regions, respectively. Therefore, the 5'UTR of GBV-C/HGV genome is highly conserved and primers deduced from this region can provideva sensitive and specific PCR assay for GBV-C/HGV RNA. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030931218&doi=10.1002%2f%28SICI%291096-9071%28199704%2951%3a4%3c284%3a%3aAID-JMV5%3e3.0.CO%3b2-1&partnerID=40&md5=2821348cc6b2171f98f063285d28e284 https://scholars.lib.ntu.edu.tw/handle/123456789/568839 |
ISSN: | 0146-6615 | DOI: | 10.1002/(SICI)1096-9071(199704)51:4<284 | SDG/關鍵字: | primer rna; article; clinical trial; controlled clinical trial; controlled study; diagnostic accuracy; diagnostic value; hepatitis g; hepatitis g virus; human; major clinical study; reverse transcription polymerase chain reaction; virus characterization; virus detection; virus genome; Base Sequence; DNA Primers; DNA, Viral; Flaviviridae; Genome, Viral; Hepatitis, Viral, Human; Humans; Molecular Sequence Data; Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity |
顯示於: | 臨床醫學研究所 |
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