https://scholars.lib.ntu.edu.tw/handle/123456789/568907
標題: | Cytogenetic studies on human liver cancer cell lines | 作者: | Chen H.-L. Chiu T.-S. PEI-JER CHEN Chen D.-S. |
公開日期: | 1993 | 卷: | 65 | 期: | 2 | 起(迄)頁: | 161-166 | 來源出版物: | Cancer Genetics and Cytogenetics | 摘要: | Karyotyping of human liver cancer cell lines and chromosome in situ hybridization of hepatitis B virus (HBV) DNA was performed in order to elucidate the possible mechanism of hepatocarcinogenesis from evidence of chromosomal changes and HBV integration patterns. HepG2-2 and HepG2-5 cell lines were HepG2 cells experimentally transfected with HBV DNA; the HCC36 cell line was derived from a hepatocellular carcinoma containing integrated HBV DNA from a Taiwanese patient. HepG2 cells, a hepatoblastoma cell line without HBV DNA integration, served as negative control. In HepG2-2, HepG2-5, and HCC36 cells, multiple integrations of HBV DNA were observed by in situ hybridization and hybridization signals occurred preferentially on certain chromosomes: 2, 5, 10, 11, 18; 7, 10, 13, 17, 18; and 4, 6, 11, 12q+, 18; respectively. In addition, a strong correlation between chromosomal changes and HBV integration was noticed in HCC36 cells, especially at chromosome 12q+. ? 1993. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027479736&doi=10.1016%2f0165-4608%2893%2990227-D&partnerID=40&md5=6c38bde04b80d720ff9ae4bc60e761ec https://scholars.lib.ntu.edu.tw/handle/123456789/568907 |
ISSN: | 0165-4608 | DOI: | 10.1016/0165-4608(93)90227-D | SDG/關鍵字: | virus dna; article; cancer cell culture; chromosome 12q; chromosome analysis; controlled study; cytogenetics; genetic transfection; hep 2 cell; hepatitis b virus; human; human cell; in situ hybridization; karyotyping; liver cancer; liver carcinogenesis; priority journal; southern blotting; Carcinoma, Hepatocellular; Chromosome Aberrations; DNA, Viral; Hepatitis B Virus; Human; In Situ Hybridization; Karyotyping; Liver Neoplasms; Support, Non-U.S. Gov't; Tumor Cells, Cultured; Hepatitis B virus |
顯示於: | 臨床醫學研究所 |
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