https://scholars.lib.ntu.edu.tw/handle/123456789/568909
標題: | Hepatitis delta virus cDNA monomer can be used in transfection experiments to initiate viral RNA replication | 作者: | Tai F.-P. PEI-JER CHEN Chang F.-L. Chen D.-S. |
公開日期: | 1993 | 卷: | 197 | 期: | 1 | 起(迄)頁: | 137-142 | 來源出版物: | Virology | 摘要: | The complementary DNA (cDNA) of hepatitis delta virus (HDV), constructed as tandem repeats under appropriate exogenous promoters, has been used to initiate viral replication in transfection experiments. Whether the structure of tandem repeats is essential has not yet been systematically examined. In this study, expression vectors containing only an HDV cDNA monomer, permutated at unique position of the genome, were shown still able to initiate viral replication. Furthermore, HDV cDNA monomer, separated from plasmid sequences by restriction enzyme digestion, also could be used to initiate viral RNA replication. The competence of HDV cDNA alone to direct viral RNA production suggested the presence of cryptic internal promoter-like elements. Such elements were actually demonstrated by the chloramphenicol acetyltransferase assay. Therefore, HDV cDNA, in contrast to that of viroids, could be used to initiate viral RNA replication in monomeric form. This observation simplified the use of HDV cDNA for studying viral biology in transfection systems. ? 1993 Academic Press Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027360814&doi=10.1006%2fviro.1993.1574&partnerID=40&md5=b6f9697dff01791ec9f3a9e4aacbc973 https://scholars.lib.ntu.edu.tw/handle/123456789/568909 |
ISSN: | 0042-6822 | DOI: | 10.1006/viro.1993.1574 |
顯示於: | 臨床醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。