https://scholars.lib.ntu.edu.tw/handle/123456789/569221
標題: | Induction of the c-jun protooncogene expression by areca nut extract and arecoline on oral mucosal fibroblasts | 作者: | Ho T.-J. CHUN-PIN CHIANG Hong C.-Y. SANG-HENG KOK Kuo Y.-S. YEN-PING KUO |
公開日期: | 2000 | 卷: | 36 | 期: | 5 | 起(迄)頁: | 432-436 | 來源出版物: | Oral Oncology | 摘要: | To investigate the mechanisms of areca quid (AQ)-induced carcinogenesis, expression of c-fos and c-jun protooncogenes was examined in human oral mucosal fibroblasts after exposure to areca nut extracts (ANE) or arecoline. We found that treatment of cells with 200 μg/ml ANE or 10 μg/ml arecoline for 1 h induced about three-fold increase in c-jun mRNA levels. This increase was transient and the level of c-jun mRNAs returned rapidly to control levels thereafter. However, ANE and arecoline did not induce c-fos mRNA expression at detectable levels. During AQ chewing, oral mucosal cells are continuously stimulated by ANE and arecoline. Persistent induction of the c-jun protooncogene by ANE and arecoline may be one of the mechanisms in the carcinogenesis of oral squamous cell carcinoma in Taiwan. Furthermore, we observed that pre-incubation of cells with either N-acetyl-cysteine [a glutathione (GSH) precursor] or L-buthionine-S,R-sulfoximine (a specific inhibitor of GSH biosynthesis) had a minimal effect on arecoline-induced c- jun expression. Therefore, arecoline-induced c-jun expression is independent of GSH depletion. (C) 2000 Elsevier Science Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033841509&doi=10.1016%2fS1368-8375%2800%2900031-2&partnerID=40&md5=b26808f7a82e002d639438183fb91946 https://scholars.lib.ntu.edu.tw/handle/123456789/569221 |
ISSN: | 1368-8375 | DOI: | 10.1016/S1368-8375(00)00031-2 | SDG/關鍵字: | acetylcysteine; arecoline; buthionine sulfoximine; glutathione; messenger RNA; protein c jun; article; betel nut; carcinogenesis; fibroblast; gene expression; gene induction; human; human cell; human tissue; mouth carcinoma; mouth mucosa; oncogene c fos; priority journal; proto oncogene; squamous cell carcinoma; Areca; Arecoline; Carcinoma, Squamous Cell; Fibroblasts; Gene Expression; Humans; Mouth Mucosa; Mouth Neoplasms; Mutagenicity Tests; Plants, Medicinal; Proto-Oncogene Proteins c-jun; Taiwan |
顯示於: | 牙醫學系 |
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