https://scholars.lib.ntu.edu.tw/handle/123456789/569323
標題: | Ludwigia octovalvis extract improves glycemic control and memory performance in diabetic mice | 作者: | Lin, Wei-Sheng Lo, Jung-Hsin Yang, Jo-Hsuan Wang, Hao-Wei SHOU-ZEN FAN Yen, Jui-Hung PEI-YU WANG |
公開日期: | 2017 | 出版社: | Elsevier Ireland Ltd | 卷: | 207 | 起(迄)頁: | 211-219 | 來源出版物: | Journal of Ethnopharmacology | 摘要: | Ethnopharmacological relevance Ludwigia octovalvis (Jacq.) P.H. Raven (Onagraceae) extracts have historically been consumed as a healthful drink for treating various conditions, including edema, nephritis, hypotension and diabetes. Aim of the study We have previously shown that Ludwigia octovalvis extract (LOE) can significantly extend lifespan and improve age-related memory deficits in Drosophila melanogaster through activating AMP-activated protein kinase (AMPK). Since AMPK has become a critical target for treating diabetes, we herein investigate the anti-hyperglycemic potential of LOE. Materials and methods Differentiated C2C12 muscle cells, HepG2 hepatocellular cells, streptozotocin (STZ)-induced diabetic mice and high fat diet (HFD)-induced diabetic mice were used to investigate the anti-hyperglycemic potential of LOE. The open field test and novel object recognition test were used to evaluate spontaneous motor activity and memory performance of HFD-induced diabetic mice. Results In differentiated C2C12 muscle cells and HepG2 hepatocellular cells, treatments with LOE and its active component (β-sitosterol) induced significant AMPK phosphorylation. LOE also enhanced uptake of a fluorescent glucose derivative (2-NBDG) and inhibited glucose production in these cells. The beneficial effects of LOE were completely abolished when an AMPK inhibitor, dorsomorphin, was added to the culture system, suggesting that LOE requires AMPK activation for its action in vitro. In streptozotocin (STZ)-induced diabetic mice, we found that both LOE and β-sitosterol induced an anti-hyperglycemic effect comparable to that of metformin, a drug that is commonly prescribed to treat diabetes. Moreover, LOE also improved glycemic control and memory performance of mice fed a HFD. Conclusions These results indicate that LOE is a potent anti-diabetic intervention that may have potential for future clinical applications. ? 2017 Elsevier Ireland Ltd |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85021313374&doi=10.1016%2fj.jep.2017.06.044&partnerID=40&md5=078a9f304a43447905edb2242ff91b21 https://scholars.lib.ntu.edu.tw/handle/123456789/569323 |
ISSN: | 0378-8741 | DOI: | 10.1016/j.jep.2017.06.044 | SDG/關鍵字: | antidiabetic agent; dorsomorphin; fluorescent dye; glucose; glucose derivative; hydroxymethylglutaryl coenzyme A reductase kinase; Ludwigia octovalvis extract; metformin; nootropic agent; plant extract; sitosterol; unclassified drug; antidiabetic agent; glucose; hydroxymethylglutaryl coenzyme A reductase kinase; plant extract; streptozocin; animal cell; animal experiment; animal model; animal tissue; antidiabetic activity; Article; cell differentiation; controlled study; enzyme activation; enzyme phosphorylation; gluconeogenesis; glucose tolerance test; glycemic control; Hep-G2 cell line; in vitro study; lipid diet; Ludwigia octovalvis; male; memory; motor activity; mouse; muscle cell; nonhuman; novel object recognition test; Onagraceae; open field test; streptozotocin-induced diabetes mellitus; animal; C57BL mouse; cell line; chemistry; drug effects; experimental diabetes mellitus; glucose blood level; human; isolation and purification; memory; metabolism; Onagraceae; pathophysiology; AMP-Activated Protein Kinases; Animals; Blood Glucose; Cell Line; Diabetes Mellitus, Experimental; Diet, High-Fat; Glucose; Hep G2 Cells; Humans; Hypoglycemic Agents; Male; Memory; Mice; Mice, Inbred C57BL; Onagraceae; Plant Extracts; Streptozocin |
顯示於: | 腦與心智科學研究所 |
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