https://scholars.lib.ntu.edu.tw/handle/123456789/569963
標題: | Induction of dental pulp fibroblast matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 gene expression by interleukin-1alpha and tumor necrosis factor-alpha through a prostaglandin-dependent pathway. | 作者: | SZE-KWAN LIN Wang C.C. Huang S. Lee J.J. CHUN-PIN CHIANG Lan W.H. Hong C.Y. |
公開日期: | 2001 | 卷: | 27 | 期: | 3 | 起(迄)頁: | 185-189 | 來源出版物: | Journal of endodontics | 摘要: | Matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) are involved in the degradation of extracellular matrix in many inflammatory diseases. Little is known regarding the expression of these mediators in dental pulp fibroblasts. The effects of proinflammatory cytokines (interleukin (IL)-1alpha and tumor necrosis factor-alpha (TNF-alpha)) and prostaglandin E2 (PGE2) on pulp fibroblast MMP-1 and TIMP-1 gene expression were investigated. Northern hybridization showed that IL-1alpha and TNF-alpha induced significant MMP-1 gene expression, with only little effect on TIMP-1 gene. Exogenous PGE2, however, upregulated TIMP-1 mRNA synthesis but not MMP-1. Concomitant addition of IL-1alpha and PGE2 or TNF-alpha and PGE2 suppressed MMP-1 mRNA production, compared with the groups treated with IL-1alpha or TNF-alpha alone. In contrast, PGE2 enhanced the upregulatory effects of TIMP-1 mRNA by IL-1alpha or TNF-alpha. Furthermore, cytokine stimulation of MMP-1 and TIMP-1 gene expressions can be enhanced or blocked by indomethacin, respectively, and reversed by exogenous PGE2. These results suggested that cytokine-stimulated MMP-1 and TIMP-1 gene expression in dental pulp fibroblasts was mediated, at least in part, by a prostaglandin-dependent pathway. The differential regulation of IL-1alpha or TNF-alpha-induced MMP-1 and TIMP-1 mRNA synthesis, as well as the direct upregulation of TIMP-1 gene expression by PGE2, also implied that prostaglandin may serve as a protective mechanism from excessive tissue breakdown during pulpitis. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035292355&doi=10.1097%2f00004770-200103000-00012&partnerID=40&md5=7620e7ff3aa535e909a7b623d3335f35 https://scholars.lib.ntu.edu.tw/handle/123456789/569963 |
ISSN: | 0099-2399 | DOI: | 10.1097/00004770-200103000-00012 | SDG/關鍵字: | autacoid; indometacin; interleukin 1; interstitial collagenase; messenger RNA; nonsteroid antiinflammatory agent; prostaglandin E2; tissue inhibitor of metalloproteinase 1; tumor necrosis factor alpha; article; comparative study; culture technique; cytology; drug effect; enzymology; fibroblast; gene expression regulation; genetics; human; Northern blotting; tooth pulp; upregulation; Anti-Inflammatory Agents, Non-Steroidal; Blotting, Northern; Cell Culture Techniques; Dental Pulp; Dinoprostone; Fibroblasts; Gene Expression Regulation, Enzymologic; Humans; Indomethacin; Inflammation Mediators; Interleukin-1; Matrix Metalloproteinase 1; RNA, Messenger; Tissue Inhibitor of Metalloproteinase-1; Tumor Necrosis Factor-alpha; Up-Regulation |
顯示於: | 臨床牙醫學研究所 |
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