https://scholars.lib.ntu.edu.tw/handle/123456789/570135
標題: | Toxicity of areca nut ingredients: Activation of CHK1/CHK2, induction of cell cycle arrest, and regulation of MMP-9 and TIMPs production in SAS epithelial cells | 作者: | Chang M.-C. Chan C.-P. Wang W.-T. BEI-EN CHANG JANG-JAER LEE Tseng S.-K. Yeung S.-Y. Hahn L.-J. JIIANG-HUEI JENG |
公開日期: | 2013 | 卷: | 35 | 期: | 9 | 起(迄)頁: | 1295-1302 | 來源出版物: | Head and Neck | 摘要: | Background There are 600 million betel quid chewers around the world. betel quid chewing is a major risk factor of oral cancer. Why betel quid components induce oral cancer is not clear. Methods Cytotoxicity of areca nut extract and arecoline (an areca nut alkaloid) to SAS oral epithelial cell line was evaluated by trypan blue dye exclusion and MTT assays. Cell cycle distribution and apoptosis was analyzed by propidium iodide staining flow cytometry. Chk1 and chk2 activation was analyzed by Pathscan phospho-enzyme-linked immunosorbent assay. Metalloproteinase-9 (MMP-9), tissue inhibitors of metalloproteinase (TIMPs) production was measured by enzyme-linked immunosorbent assay. Results Areca nut extract (800 μg/mL) and arecoline (>0.4 mmol/L) caused cell death, apoptosis, and cell cycle arrest of SAS cells. Areca nut extract and arecoline stimulated Chk1 and Chk2 phosphorylation in SAS cells. Areca nut extract stimulated cellular MMP-9 but suppressed TIMP-1 and TIMP-2 production. Conclusions Areca nut components activate Chk1/Chk2, alter cell cycle regulation/apoptosis, MMP-9, and TIMPs production, contributing to the pathogenesis of oral carcinogenesis. ? 2012 Wiley Periodicals, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883055477&doi=10.1002%2fhed.23119&partnerID=40&md5=c5ed0d3d83b93490729e782122d39067 https://scholars.lib.ntu.edu.tw/handle/123456789/570135 |
ISSN: | 1043-3074 | DOI: | 10.1002/hed.23119 | SDG/關鍵字: | arecoline; checkpoint kinase 1; checkpoint kinase 2; gelatinase B; tissue inhibitor of metalloproteinase; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2; apoptosis; article; betel nut; carcinogenesis; cell cycle arrest; controlled study; cytotoxicity; enzyme activation; enzyme linked immunosorbent assay; enzyme phosphorylation; enzyme regulation; enzyme synthesis; epithelium cell; genotoxicity; mouth cancer; priority journal; areca nut; arecoline; betel quid; checkpoint kinase; chemical carcinogenesis; metalloproteinase-9; tissue inhibitors of metalloproteinases; Areca; Arecoline; Cell Cycle; Cells, Cultured; Checkpoint Kinase 2; Enzyme Activation; Enzyme-Linked Immunosorbent Assay; Epithelial Cells; Flow Cytometry; Humans; Matrix Metalloproteinase 9; Nuts; Plant Extracts; Protein Kinases; Tissue Inhibitor of Metalloproteinases; Tongue Neoplasms |
顯示於: | 臨床牙醫學研究所 |
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