https://scholars.lib.ntu.edu.tw/handle/123456789/570189
標題: | Inducing the cell cycle arrest and apoptosis of oral KB carcinoma cells by hydroxychavicol: Roles of glutathione and reactive oxygen species | 作者: | Chang M.C. Uang B.J. Wu H.L. JANG-JAER LEE Hahn L.J. Jeng J.H. |
關鍵字: | Apoptosis; Betel quid; Cell cycle; Hydroxychavicol; Piper betle; Reactive oxygen species; Redox status | 公開日期: | 2002 | 卷: | 135 | 期: | 3 | 起(迄)頁: | 619-630 | 來源出版物: | British Journal of Pharmacology | 摘要: | 1. Hydroxychavicol (HC; 10-50 μM), a betel leaf component, was found to suppress the 2% H2O2-induced lucigenin chemiluminescence for 53-75%. HC (0.02-2μM) was also able to trap superoxide radicals generated by a xanthine/xanthine oxidase system with 38-94% of inhibition. Hydroxyl radicals-induced PUC18 plasmid DNA breaks was prevented by HC (1.6-16 μM). 2. A 24-h exposure of KB cells to HC (0.5, 1 mM) resulted in 54-74% cell death as analysed by a 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. HC (10, 50 μM) further suppressed the growth of KB cells (15 and 76%, respectively). Long-term colony formation of KB cells was inhibited by 51% with 10 μM HC. 3. Pretreatment of KB cells with 100 μM HC inhibited the attachment of KB cells to type I collagen and fibronectin by 59 and 29%, respectively. 4. Exposure of KB cells to 0.1 mM HC for 24 h resulted in cell cycle arrest at late S and G2/M phase. Increasing the HC concentration to 0.25 and 0.5 mM led to apoptosis as revealed by detection of sub-G0/G1 peaks with a concomitant decrease in the number of cells residing in late S and G2/M phase. 5. Inducing the apoptosis of KB cells by HC was accompanied by marked depletion in reduced form of GSH (>0.2 mM) and the increasing of reactive oxygen species production (>0.1 mM) as analysed by CMF- and DCF-single cell fluorescence flow cytometry. 6. These results indicate that HC exerts antioxidant property at low concentration. HC also inhibits the growth, adhesion and cell cycle progression of KB cells, whereas its induction of KB cell apoptosis (HC>0.1 mM) was accompanied by cellular redox changes. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036176605&doi=10.1038%2fsj.bjp.0704492&partnerID=40&md5=ed53010549d6a26d6d5024fa66df0ef0 https://scholars.lib.ntu.edu.tw/handle/123456789/570189 |
ISSN: | 0007-1188 | DOI: | 10.1038/sj.bjp.0704492 | SDG/關鍵字: | 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; collagen type 1; fibronectin; glutathione; hydrogen peroxide; hydroxychavicol; hydroxyl radical; lucigenin; plant extract; plasmid DNA; reactive oxygen metabolite; superoxide; unclassified drug; xanthine; xanthine oxidase; antioxidant activity; apoptosis; article; betel nut; cancer inhibition; cell adhesion; cell cycle; cell cycle G0 phase; cell cycle G1 phase; cell cycle G2 phase; cell cycle S phase; cell growth; colony formation; concentration response; DNA strand breakage; enzyme inhibition; human; human cell; mouth carcinoma; oxidation reduction reaction; priority journal; Anticarcinogenic Agents; Antineoplastic Agents; Apoptosis; Areca; Cell Adhesion; Cell Cycle; Cell Size; Collagen; Dose-Response Relationship, Drug; Eugenol; Fibronectins; Glutathione; Growth Inhibitors; Humans; KB Cells; Oxidative Stress; Reactive Oxygen Species |
顯示於: | 臨床牙醫學研究所 |
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