|Title:||Inhibition of periodontitis induction using a stimuli-responsive hydrogel carrying naringin||Authors:||PO-CHUN CHANG
|Keywords:||Chitosan; Drug delivery systems; Flavonoids; Inflammation; Periodontitis||Issue Date:||2017||Publisher:||American Academy of Periodontology||Journal Volume:||88||Journal Issue:||2||Start page/Pages:||190-196||Source:||Journal of Periodontology||Abstract:||
Background: Developing a drug carrier with favorable handling characteristics that can respond to environmental changes after inflammation, such as pH changes, may be beneficial for treating periodontitis. This study aims to investigate the preclinical feasibility of using naringin, a naturally derived polymethoxylated flavonoid compound with antiinflammatory properties, to inhibit periodontitis induction via a thermogelling and pH-responsive injectable hydrogel. Methods: The hydrogel was made of amphipathic carboxymethyl-hexanoyl chitosan (CHC), β-glycerol phosphate (β-GP), and glycerol. Thermogelling and pH-responsive characteristics of the hydrogel, as well as cell viability after treatment with the hydrogel containing naringin, were evaluated in vitro. Hydrogel was subgingivally delivered when experimental periodontitis was induced in vivo, and therapeutic effect was evaluated with microcomputed tomography imaging, histology, and expression of inflammation-associated genes, including toll-like receptor (TLR)2, the receptor for advanced glycation end products (RAGE), myeloid differentiation primary response gene-88, and tumor necrosis factor (TNF)-α. Results: The hydrogel was consistently fluidic at 4°C but rapidly gelled at 37°C. Release of naringin was faster at pH 5.5 to 6.5, and viability was significantly promoted by treatment with 0.85% naringin. Naringin-carrying CHC-β-GP-glycerol hydrogel sites showed significantly reduced periodontal bone loss (P <0.05) and inflammatory infiltration (P <0.01) as well as significantly downregulated TLR2 (P <0.05), RAGE (P <0.01), and TNF-α (P <0.05) relative to the sites with experimental periodontitis alone. Conclusion: Naringin-carrying CHC-β-GP-glycerol colloidal hydrogel can be used to inhibit induction of experimental periodontitis with favorable handling and inflammation-responsive characteristics. ? 2017 American Academy of Periodontology. All rights reserved.
|ISSN:||0022-3492||DOI:||10.1902/jop.2016.160189||SDG/Keyword:||aurantiin; drug carrier; flavanone derivative; polyethylene glycol dimethacrylate hydrogel; animal; C57BL mouse; cell culture; cell survival; chemistry; cytology; diagnostic imaging; disease model; human; male; micro-computed tomography; mouse; periodontal ligament; periodontitis; Animals; Cell Survival; Cells, Cultured; Disease Models, Animal; Drug Carriers; Flavanones; Humans; Hydrogel, Polyethylene Glycol Dimethacrylate; Male; Mice; Mice, Inbred C57BL; Periodontal Ligament; Periodontitis; X-Ray Microtomography
|Appears in Collections:||臨床牙醫學研究所|
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