https://scholars.lib.ntu.edu.tw/handle/123456789/572880
標題: | Rapid Quantification of Gut Microbial Short-Chain Fatty Acids by pDART-MS | 作者: | LEE-YAN SHEEN CHENG-CHIH HSU |
關鍵字: | Diagnosis; Drug products; Fermentation; Mammals; Mass spectrometry; Volatile fatty acids; Biological samples; Direct analysis in real time; Fermentation products; High throughput screening; Industrial quality; Limit of detection; Short chain fatty acids (SCFAs); Short-chain fatty acids; Quality control | 公開日期: | 2020 | 卷: | 92 | 期: | 22 | 起(迄)頁: | 14892-14897 | 來源出版物: | Analytical Chemistry | 摘要: | Short-chain fatty acids (SCFAs) are small molecules ubiquitous in nature. In mammalian guts, SCFAs are mostly produced by anaerobic intestinal microbiota through the fermentation of dietary fiber. Levels of microbe-derived SCFAs are closely relevant to human health status and indicative to gut microbiota dysbiosis. However, the quantification of SCFA using conventional chromatographic approaches is often time consuming, thus limiting high-throughput screening tests. Herein, we established a novel method to quantify SCFAs by coupling amidation derivatization of SCFAs with paper-loaded direct analysis in real time mass spectrometry (pDART-MS). Remarkably, SCFAs of a biological sample were quantitatively determined within a minute using the pDART-MS platform, which showed a limit of detection at the μM level. This platform was applied to quantify SCFAs in various biological samples, including feces from stressed rats, sera of patients with kidney disease, and fermentation products of metabolically engineered cyanobacteria. Significant differences in SCFA levels between different groups of biological practices were promptly revealed and evaluated. As there is a burgeoning demand for the analysis of SCFAs due to an increasing academic interest of gut microbiota and its metabolism, this newly developed platform will be of great potential in biological and clinical sciences as well as in industrial quality control. ? 2020 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096893088&doi=10.1021%2facs.analchem.0c03862&partnerID=40&md5=c70dd267dc9d8216b6d2e3aec7ee3f75 https://scholars.lib.ntu.edu.tw/handle/123456789/572880 |
ISSN: | 32700 | DOI: | 10.1021/acs.analchem.0c03862 | SDG/關鍵字: | Diagnosis; Drug products; Fermentation; Mammals; Mass spectrometry; Volatile fatty acids; Biological samples; Direct analysis in real time; Fermentation products; High throughput screening; Industrial quality; Limit of detection; Short chain fatty acids (SCFAs); Short-chain fatty acids; Quality control |
顯示於: | 食品科技研究所 |
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