https://scholars.lib.ntu.edu.tw/handle/123456789/572897
標題: | 3′-Hydroxypterostilbene Inhibits 7,12-Dimethylbenz[a]anthracene (DMBA)/12-O-Tetradecanoylphorbol-13-Acetate (TPA)-Induced Mouse Skin Carcinogenesis | 作者: | Lee P.-S Chiou Y.-S Chou P.-Y Nagabhushanam K Ho C.-T MIN-HSIUNG PAN |
關鍵字: | 3' hydroxypterostilbene; 7,12 dimethylbenz[a]anthracene; cyclin B1; cyclin dependent kinase 1; cycline; cyclooxygenase 2; cytochrome P450 1A1; cytochrome P450 1B1; dimethylbenz[a]anthracene; gelatinase B; ornithine decarboxylase; phorbol 13 acetate 12 myristate; pterostilbene; unclassified drug; 3'-hydroxypterostilbene; antineoplastic agent; carcinogen; cyclooxygenase 2; nonsteroid antiinflammatory agent; ornithine decarboxylase; Ptgs2 protein, mouse; stilbene derivative; animal experiment; animal model; animal tissue; antiinflammatory activity; antineoplastic activity; antitumorigenic activity; Article; cell infiltration; cell viability; controlled study; dermatitis; down regulation; epidermis hyperplasia; epithelium hyperplasia; female; gene expression; human; human cell; immunoreactivity; keratinocyte; leukocyte; mitosis; mouse; nonhuman; priority journal; protein expression; skin carcinogenesis; skin tumor; tumor growth; tumor weight; animal; drug effect; drug eruption; gene expression regulation; Institute for Cancer Research mouse; metabolism; pathology; skin tumor; topical drug administration; 9,10-Dimethyl-1,2-benzanthracene; Administration, Topical; Animals; Anti-Inflammatory Agents, Non-Steroidal; Anticarcinogenic Agents; Carcinogens; Cyclooxygenase 2; Drug Eruptions; Female; Gene Expression Regulation; Humans; Keratinocytes; Mice, Inbred ICR; Ornithine Decarboxylase; Skin Neoplasms; Stilbenes; Tetradecanoylphorbol Acetate | 公開日期: | 2021 | 卷: | 81 | 來源出版物: | Phytomedicine | 摘要: | Background: A natural pterostilbene analogue isolated from the herb Sphaerophysa salsula, 3′-hydroxypterostilbene (HPSB), exhibits antiproliferative activity in several cancer cell lines; however, the inhibitory effects of HPSB on skin carcinogenesis remains unclear. Purpose: The aim of this study was to evaluate the inhibitory effects of HPSB on two-stage skin carcinogenesis in mice and its potential mechanism. Study Design and Methods: This study investigated the anti-inflammatory and anti-tumor effects of HPSB in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated acute skin inflammation and 7,12-dimethylbenz[a]anthracene (DMBA)/TPA-induced two-stage skin carcinogenesis model. In addition, the effects of HPSB on the modulation of the phase I and phase II metabolizing enzymes in the DMBA-induced HaCaT cell model were investigated. Results: The results provide evidence that topical treatment with HPSB significantly inhibits TPA-induced epidermal hyperplasia and leukocyte infiltration through the down-regulation of cyclooxygenase-2 (COX-2), matrix metalloprotein-9 (MMP-9), and ornithine decarboxylase (ODC) protein expression in mouse skin. Furthermore, HPSB suppresses DMBA/TPA-induced skin tumor incidence and multiplicity via the inhibition of proliferating cell nuclear antigen (PCNA), Cyclin B1 and cyclin-dependent kinase 1 (CDK1) expression in the two-stage skin carcinogenesis model. In addition, pretreatment with HPSB markedly reduces DMBA-induced cytochrome P450 1A1 (CYP1A1) and cytochrome P450 1B1 (CYP1B1) gene expression in human keratinocytes; however, HPSB does not significantly affect the gene expression of the phase II enzymes. Conclusion: This is the first study to show that topical treatment with HPSB prevents mouse skin tumorigenesis. Overall, our study suggests that natural HPSB may serve as a novel chemopreventive agent capable of preventing carcinogen activation and inflammation-associated tumorigenesis. ? 2020 |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097479476&doi=10.1016%2fj.phymed.2020.153432&partnerID=40&md5=4855bbfb59846c86f69b41297ac4937f https://scholars.lib.ntu.edu.tw/handle/123456789/572897 |
ISSN: | 9447113 | DOI: | 10.1016/j.phymed.2020.153432 | SDG/關鍵字: | 3' hydroxypterostilbene; 7,12 dimethylbenz[a]anthracene; cyclin B1; cyclin dependent kinase 1; cycline; cyclooxygenase 2; cytochrome P450 1A1; cytochrome P450 1B1; dimethylbenz[a]anthracene; gelatinase B; ornithine decarboxylase; phorbol 13 acetate 12 myr |
顯示於: | 食品科技研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。