https://scholars.lib.ntu.edu.tw/handle/123456789/572960
標題: | Penaeus vannamei serine proteinase inhibitor 7 (LvSerpin7) acts as an immune brake by regulating the proPO system in AHPND-affected shrimp | 作者: | Apitanyasai K Chang C.-C Ng T.H Ng Y.S Liou J.-H Lo C.-F SHIH-SHUN LIN Wang H.-C. |
關鍵字: | messenger RNA; monophenol monooxygenase; serine proteinase inhibitor; serine proteinase inhibitor 7; unclassified drug; acute hepatopancreatic necrosis disease; adult; animal tissue; Article; bacterial strain; bacterial virulence; blood cell; controlled study; enzyme activity; hemolymph; immunity; in vitro study; liver necrosis; mortality; nonhuman; pancreas necrosis; Penaeus vannamei; priority journal; protein expression; shrimp; stomach; Vibrio parahaemolyticus | 公開日期: | 2020 | 卷: | 106 | 來源出版物: | Developmental and Comparative Immunology | 摘要: | Acute hepatopancreatic necrosis disease (AHPND) is a recently emerged disease in aqua cultured shrimp that is caused by virulent strains of Vibrio parahaemolyticus (VP). Our previous study used transcriptomics to identify key pathogenic factors in the stomach of AHPND-infected shrimp (Litopenaeus vannamei), and here we used a different subset of the same data to construct a gene-to-gene expression correlation network to identify immune-responsive genes. LvSerpin7 was found to have the highest number of correlations after infection, and it also showed a significant increase in mRNA expression. LvSerpin7 is expressed in all tissues but its expression levels are highest in hemocytes. After successfully silencing LvSerpin7 transcript prior to AHPND challenge, mortality was significantly increased relative to the controls and reached 100% within 36 h post infection. Compared to the controls, the phenoloxidase (PO) activity also increased in both hemolymph and stomach. Recombinant LvSerpin7 inhibited shrimp PO activity in vitro, and we also found that rLvSerpin7 inhibited the growth of AHPND-causing bacteria. These results suggest that LvSerpin7 might reduce the toxic effects that result from unregulated activation of the PO defense system by AHPND-causing bacteria. ? 2019 Elsevier Ltd |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077462227&doi=10.1016%2fj.dci.2019.103600&partnerID=40&md5=21caa2dc180f89622f76df4d81ef8654 https://scholars.lib.ntu.edu.tw/handle/123456789/572960 |
ISSN: | 0145305X | DOI: | 10.1016/j.dci.2019.103600 |
顯示於: | 生物科技研究所 |
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