https://scholars.lib.ntu.edu.tw/handle/123456789/573156
標題: | Cinnamtannin B1 attenuates rosacea-like signs via inhibition of pro-inflammatory cytokine production and down-regulation of the MAPK pathway | 作者: | Kan H.-L CHIA-CHI WANG Cheng Y.-H Yang C.-L Chang H.-S Chen I.-S Lin Y.-C. |
關鍵字: | bovine serum albumin; cathelicidin; cetrimide; chemokine; cinnamtannin B1; collagenase 3; cyclooxygenase 2; cytokine; dodecyl sulfate sodium; formylpeptide receptor like 1; horseradish peroxidase; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 10; interleukin 1beta; interleukin 6; interleukin 8; inulin; isoflurane; ivermectin; macrophage inflammatory protein 2; messenger RNA; metronidazole; mitogen activated protein kinase; myeloperoxidase; polyclonal antibody; polyvinylidene fluoride; proteinase inhibitor; reactive oxygen metabolite; reagent; ropocamptide; stress activated protein kinase; tannin derivative; tumor necrosis factor; unclassified drug; vascular cell adhesion molecule 1; animal cell; animal experiment; animal model; antiinflammatory activity; Article; Bacillus subtilis; carbon nuclear magnetic resonance; cell infiltration; cell viability; chemoluminescence; controlled study; cytokine production; DNA extraction; down regulation; enzyme linked immunosorbent assay; erythema; fetal calf serum; gene expression; histology; histopathology; human; human cell; human tissue; immunohistochemistry; inflammation; innate immunity; keratinocyte; macrophage; male; mouse; neutrophil chemotaxis; nonhuman; Propionibacterium acnes; protein expression; protein phosphorylation; proton nuclear magnetic resonance; radioimmunoprecipitation; real time reverse transcription polymerase chain reaction; RNA isolation; Staphylococcus aureus; Western blotting | 公開日期: | 2020 | 卷: | 8 | 來源出版物: | PeerJ | 摘要: | Background. Rosacea is a common inflammatory disease of facial skin. Dysregulation of innate immunity with enhanced inflammation and increased abundance of LL-37 at the epidermal site is a characteristic feature of rosacea. Cinnamtannin B1 (CB1) is a condensed tannin with anti-inflammatory and anti-microbial activities. The aims of the study were to evaluate the potential of CB1 as a therapy for rosacea and to characterize the potential mechanisms of action. Methods. We intraperitoneally administered 20 mg/kg CB1 once daily for 2 days into the LL-37-induced mouse model of rosacea. The effects of CB1 in vivo were evaluated by the observations of lesions, histology, immunohistochemistry, and the transcription and translation of pro-inflammatory cytokines and chemokines. Human keratinocyte HaCaT and monocyte THP-1 were used to characterize the effects of CB1 on LL-37-induced inflammation in vitro. The changes in pro-inflammatory chemokine interleukin-8 (IL-8) were quantitated by enzyme-linked immunosorbent assay (ELISA), and the expressions of genes involved were determined by Western blotting. Results. CB1 attenuated local redness, inflammation, and neutrophil recruitment in the mouse model of rosacea in vivo. CB1 suppressed myeloperoxidase (MPO) and macrophage inflammatory protein 2 (MIP-2) production, a functional homolog of interleukin-8 (IL-8), at the lesions. In vitro experiments confirmed that CB1 reversed the LL-37-induced IL-8 production in human keratinocytes HaCaT and monocyte THP-1 cells. CB1 inhibited IL-8 production through downregulating the phosphorylation of extracellular signal-regulated kinase (ERK) in the mitogen-activated protein kinase (MAPK) pathway. Conclusion. CB1 attenuated LL-37-induced inflammation, specifically IL-8 production, through inhibiting the phosphorylation of ERK. CB1 has potential as a treatment for rosacea. Copyright 2020 Kan et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098129104&doi=10.7717%2fpeerj.10548&partnerID=40&md5=013b448557a74fdc693745a5f02a4557 https://scholars.lib.ntu.edu.tw/handle/123456789/573156 |
ISSN: | 21678359 | DOI: | 10.7717/peerj.10548 | SDG/關鍵字: | bovine serum albumin; cathelicidin; cetrimide; chemokine; cinnamtannin B1; collagenase 3; cyclooxygenase 2; cytokine; dodecyl sulfate sodium; formylpeptide receptor like 1; horseradish peroxidase; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 10; interleukin 1beta; interleukin 6; interleukin 8; inulin; isoflurane; ivermectin; macrophage inflammatory protein 2; messenger RNA; metronidazole; mitogen activated protein kinase; myeloperoxidase; polyclonal antibody; polyvinylidene fluoride; proteinase inhibitor; reactive oxygen metabolite; reagent; ropocamptide; stress activated protein kinase; tannin derivative; tumor necrosis factor; unclassified drug; vascular cell adhesion molecule 1; animal cell; animal experiment; animal model; antiinflammatory activity; Article; Bacillus subtilis; carbon nuclear magnetic resonance; cell infiltration; cell viability; chemoluminescence; controlled study; cytokine production; DNA extraction; down regulation; enzyme linked immunosorbent assay; erythema; fetal calf serum; gene expression; histology; histopathology; human; human cell; human tissue; immunohistochemistry; inflammation; innate immunity; keratinocyte; macrophage; male; mouse; neutrophil chemotaxis; nonhuman; Propionibacterium acnes; protein expression; protein phosphorylation; proton nuclear magnetic resonance; radioimmunoprecipitation; real time reverse transcription polymerase chain reaction; RNA isolation; Staphylococcus aureus; Western blotting |
顯示於: | 獸醫學系 |
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