https://scholars.lib.ntu.edu.tw/handle/123456789/573171
標題: | Facile Transformation of Murine and Human Primary Dendritic Cells into Robust and Modular Artificial Antigen-Presenting Systems by Intracellular Hydrogelation | 作者: | Lin J.-C HUI-WEN CHEN et al. |
關鍵字: | Antigens; Expansion; Gelation; Hydrogels; Mammals; Morphology; Polyethylene glycols; T-cells; Antigen presenting cells; Artificial antigen; Bottom up approach; Cancer immunotherapy; Cellular morphology; Dendritic cells (DCs); Polyethylene glycol diacrylate; Synthetic substrates; Cytology | 公開日期: | 2021 | 來源出版物: | Advanced Materials | 摘要: | The growing enthusiasm for cancer immunotherapies and adoptive cell therapies has prompted increasing interest in biomaterials development mimicking natural antigen-presenting cells (APCs) for T-cell expansion. In contrast to conventional bottom-up approaches aimed at layering synthetic substrates with T-cell activation cues, transformation of live dendritic cells (DCs) into artificial APCs (aAPCs) is demonstrated herein using a facile and minimally disruptive hydrogelation technique. Through direct intracellular permeation of poly(ethylene glycol) diacrylate (PEG-DA) hydrogel monomer and UV-activated radical polymerization, intracellular hydrogelation is rapidly accomplished on DCs with minimal influence on cellular morphology and surface antigen display, yielding highly robust and modular cell–gel hybrid constructs amenable to peptide antigen exchange, storable by freezing and lyophilization, and functionalizable with cytokine-releasing carriers for T-cell modulation. The DC-derived aAPCs are shown to induce prolonged T-cell expansion and improve anticancer efficacy of adoptive T-cell therapy in mice compared to nonexpanded control T cells, and the gelation technique is further demonstrated to stabilize primary DCs derived from human donors. The work presents a versatile approach for generating a new class of cell-mimicking biomaterials and opens new venues for immunological interrogation and immunoengineering. ? 2021 Wiley-VCH GmbH |
URI: | https://www.scopus.com/record/display.uri?eid=2-s2.0-85107202090&origin=resultslist https://scholars.lib.ntu.edu.tw/handle/123456789/573171 |
ISSN: | 9359648 | DOI: | 10.1002/adma.202101190 | SDG/關鍵字: | Antigens; Expansion; Gelation; Hydrogels; Mammals; Morphology; Polyethylene glycols; T-cells; Antigen presenting cells; Artificial antigen; Bottom up approach; Cancer immunotherapy; Cellular morphology; Dendritic cells (DCs); Polyethylene glycol diacrylat |
顯示於: | 獸醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。