https://scholars.lib.ntu.edu.tw/handle/123456789/573244
標題: | Mycolactone-producing Mycobacterium marinum infection in captive Hong Kong warty newts and pathological evidence of impaired host immune function | 作者: | Li W.-T HUI-WEN CHANG VICTOR FEI PANG Wang F.-I Liu C.-H Chen T.-Y Guo J.-C Wada T CHIAN-REN JENG |
關鍵字: | abundance; bacterium; genetic marker; immune response; lesion; mass mortality; polymerase chain reaction; protozoan; skin disorder; toxin; China; Hong Kong; Taipei; Taiwan; Amphibia; Bacilli (class); Bacteria (microorganisms); Mycobacterium marinum; Oomycetes; Paramesotriton; Paramesotriton hongkongensis; Ranavirus; Salamandridae; Saprolegnia; bacterial DNA; macrolide; mycolactone; animal; atypical mycobacteriosis; genetics; immunology; metabolism; microbiology; mortality; Mycobacterium marinum; nucleotide sequence; Salamandridae; veterinary; Animals; Base Sequence; DNA, Bacterial; Macrolides; Mycobacterium Infections, Nontuberculous; Mycobacterium marinum; Salamandridae | 公開日期: | 2017 | 卷: | 123 | 期: | 3 | 起(迄)頁: | 239-249 | 來源出版物: | Diseases of Aquatic Organisms | 摘要: | A mass mortality event of captive Hong Kong warty newts Paramesotriton hong - kongensis with non-granulomatous necrotic lesions occurred in Taipei Zoo, Taiwan, in 2014. Clinically, the sick newts were lethargic and often covered with water mold Saprolegnia sp. on the skin of the body trunk or extremities. Predominant pathological findings were multifocal nongranulomatous necrotic lesions in the liver, spleen, and kidneys and severe skin infection with Sapro legnia sp., with deep invasion and involvement of underlying muscles. The possibility of ranavirus infection was ruled out by negative PCR results. Unexpectedly, abundant intralesional acid-fast positive bacilli were found in the necrotic lesions of the liver, spleen, and kidney in all 14 sick newts. PCR targeting the hsp65, ITS region, and partial 16S rRNA genes was performed, and the sequence identity from amplified amplicons of hsp65 and partial 16S rRNA genes was 100% identical to that of the corresponding gene fragment of Mycobacterium marinum. Further molecular investigations demonstrated that the current M. marinum was a mycolactone-producing myco bacterium with the presence of esxA/esxB genes. Mycolactone is a plasmid-encoded, immunosuppressive, and cytotoxic toxin. The possible immunosuppression phenomenon characterized by systemic non-granulomatous necrotic lesions caused by M. marinum and the unusual deep invasive infection caused by water mold might be associated with the immunosuppressive effect of mycolactone. Therefore, it should be noted that non-granulomatous necrotic lesions in amphibians can be caused not only by ranavirus infection but also by mycobacteriosis. ? 2017 Inter-Research. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020445736&doi=10.3354%2fdao03092&partnerID=40&md5=91a24b8c9b40209376379eefa56174ff https://scholars.lib.ntu.edu.tw/handle/123456789/573244 |
ISSN: | 1775103 | DOI: | 10.3354/dao03092 |
顯示於: | 分子暨比較病理生物學研究所 |
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