|Title:||Sensitive and Specific Cadmium Biosensor Developed by Reconfiguring Metal Transport and Leveraging Natural Gene Repositories||Authors:||He M.-Y
|Keywords:||Agricultural robots; Bioassay; Biosensors; Cost effectiveness; Economic and social effects; Escherichia coli; Fluorescence; Irrigation; Metal ions; mHealth; Toxicity; Transcription; Concentration ranges; Engineering solutions; Fluorescent intensity; Green fluorescent protein; Heavy metal toxicity; Metal transport systems; Sensitivity and specificity; Whole-cell biosensors; Cadmium; cadmium; metal; ecosystem; Escherichia coli; genetic procedures; genetics; human; Biosensing Techniques; Cadmium; Ecosystem; Escherichia coli; Humans; Metals||Issue Date:||2021||Journal Volume:||6||Journal Issue:||3||Start page/Pages:||995-1002||Source:||ACS Sensors||Abstract:||
Whole-cell biosensors are useful for monitoring heavy metal toxicity in public health and ecosystems, but their development has been hindered by intrinsic trade-offs between sensitivity and specificity. Here, we demonstrated an effective engineering solution by building a sensitive, specific, and high-response biosensor for carcinogenic cadmium ions. We genetically programmed the metal transport system of Escherichia coli to enrich intracellular cadmium ions and deprive interfering metal species. We then selected 16 cadmium-sensing transcription factors from the GenBank database and tested their reactivity to 14 metal ions in the engineered E. coli using the expression of the green fluorescent protein as the readout. The resulting cadmium biosensor was highly specific and showed a detection limit of 3 nM, a linear increase in fluorescent intensities from 0 to 200 nM, and a maximal 777-fold signal change. Using this whole-cell biosensor, a smartphone, and low-tech equipment, we developed a simple assay capable of measuring cadmium ions at the same concentration range in irrigation water and human urine. This method is user-friendly and cost-effective, making it affordable to screen large amounts of samples for cadmium toxicity in agriculture and medicine. Moreover, our work highlights natural gene repositories as a treasure chest for bioengineering. ?
Agricultural robots; Bioassay; Biosensors; Cost effectiveness; Economic and social effects; Escherichia coli; Fluorescence; Irrigation; Metal ions; mHealth; Toxicity; Transcription; Concentration ranges; Engineering solutions; Fluorescent intensity; Green
|Appears in Collections:||生命科學系|
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