https://scholars.lib.ntu.edu.tw/handle/123456789/573317
標題: | Chitosan 3D cell culture system promotes naïve-like features of human induced pluripotent stem cells: A novel tool to sustain pluripotency and facilitate differentiation | 作者: | Chang P.-H Chao H.-M Edward CHERN SHAN-HUI HSU |
關鍵字: | Biocompatibility; Cell culture; Chitosan; Cost effectiveness; Diagnosis; Recombinant proteins; Biocompatible polysaccharides; Developmental stage; Human-induced pluripotent stem cells; Manufacturing process; Mesenchymal stem cell; Physiological activity; Pluripotent stem cells; Three-dimensional (3d) cell culture; Stem cells; biomaterial; chitosan; vitronectin; animal cell; Article; cell density; cell differentiation; cell expansion; cell membrane; cell proliferation; cell structure; cell survival; controlled study; developmental stage; germ layer; human; human cell; induced pluripotent stem cell; mouse; nonhuman; priority journal; SCID mouse; spheroid cell; three dimensional cell culture; cell culture technique; cell differentiation; pluripotent stem cell; Cell Culture Techniques; Cell Differentiation; Chitosan; Humans; Induced Pluripotent Stem Cells; Pluripotent Stem Cells | 公開日期: | 2021 | 出版社: | Elsevier Ltd | 卷: | 268 | 期: | Article number 120575 | 來源出版物: | Biomaterials | 摘要: | A simplified and cost-effective culture system for maintaining the pluripotency of human induced pluripotent stem cells (hiPSCs) is crucial for stem cell applications. Although recombinant protein-based feeder-free hiPSC culture systems have been developed, their manufacturing processes are expensive and complicated, which hinders hiPSC technology progress. Chitosan, a versatile biocompatible polysaccharide, has been reported as a biomaterial for three-dimensional (3D) cell culture system that promotes the physiological activities of mesenchymal stem cells and cancer cells. In the current study, we demonstrated that chitosan membranes sustained proliferation and pluripotency of hiPSCs in long-term culture (up to 365 days). Moreover, using vitronectin as the comparison group, the pluripotency of hiPSCs grown on the membranes was altered into a na?ve-like state, which, for pluripotent stem cells, is an earlier developmental stage with higher stemness. On the chitosan membranes, hiPSCs self-assembled into 3D spheroids with an average diameter of ~100 μm. These hiPSC spheroids could be directly differentiated into lineage-specific cells from the three germ layers with 3D structures. Collectively, chitosan membranes not only promoted the na?ve pluripotent features of hiPSCs but also provided a novel 3D differentiation platform. This convenient biomaterial-based culture system may enable the effective expansion and accessibility of hiPSCs for regenerative medicine, disease modeling, and drug screening. ? 2020 Elsevier Ltd |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097713826&doi=10.1016%2fj.biomaterials.2020.120575&partnerID=40&md5=2ecb27c4a61b7e17756f00bf843ce4ef https://scholars.lib.ntu.edu.tw/handle/123456789/573317 |
ISSN: | 1429612 | DOI: | 10.1016/j.biomaterials.2020.120575 | SDG/關鍵字: | Biocompatibility; Cell culture; Chitosan; Cost effectiveness; Diagnosis; Recombinant proteins; Biocompatible polysaccharides; Developmental stage; Human-induced pluripotent stem cells; Manufacturing process; Mesenchymal stem cell; Physiological activity; |
顯示於: | 生化科技學系 |
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