https://scholars.lib.ntu.edu.tw/handle/123456789/574268
標題: | All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures | 作者: | Huang, C. Tai, C.-Y. Yang, K.-P. Chang, W.-K. Hsu, K.-J. Hsiao, C.-C. Wu, S.-C. Lin, Y.-Y. Chiang, A.-S. Chu, S.-W. SHI-WEI CHU |
關鍵字: | Biological Sciences Research Methodologies; Biological Sciences Tools; Neuroscience; Techniques in Neuroscience | 公開日期: | 2019 | 卷: | 22 | 起(迄)頁: | 133-146 | 來源出版物: | iScience | 摘要: | Neuroscience; Techniques in Neuroscience; Biological Sciences Research Methodologies; Biological Sciences Tools ? 2019 The Author(s)All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon point stimulation and two-photon high-speed volumetric recordings with a tunable acoustic gradient-index (TAG) lens. We demonstrated the platform effectiveness by studying the anterior visual pathway (AVP) of Drosophila. We achieved functional observation of spatiotemporal coding and the strengths of calcium-sensitive connections between anterior optic tubercle (AOTU) sub-compartments and >70 tightly assembled 2-μm bulb (BU) microglomeruli in 3D coordinates with a single trial. Our work aids the establishment of in vivo 3D functional connectomes in neuron-dense brain areas. ? 2019 The Author(s) |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85075210323&partnerID=40&md5=a457ff49fc8d681aec206f2b802035a2 https://scholars.lib.ntu.edu.tw/handle/123456789/574268 |
DOI: | 10.1016/j.isci.2019.11.011 |
顯示於: | 物理學系 |
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