https://scholars.lib.ntu.edu.tw/handle/123456789/575506
標題: | Modulation of Anti-Adhesion Molecule MUC-1 Is Associated with Arctiin-Induced Growth Inhibition in PC-3 Cells | 作者: | Huang D.-M. JIH-HWA GUH SHIH-CHIEH CHUEH Teng C.-M. |
公開日期: | 2004 | 卷: | 59 | 期: | 3 | 起(迄)頁: | 260-267 | 來源出版物: | Prostate | 摘要: | BACKGROUND. Lignans have been reported to possess anti-tumor activity in various cancer cells. However, their anticancer effects in human prostate cancer have not been well established. Here, we examine the effect of arctiin, a lignan compound, on growth regulation in prostate cancer PC-3 cells. We postulated that arctiin modulates the attachment/detachment of PC-3 cells and we investigated the role of arctiin on MUC-1 expression. METHODS. The effect of arctiin on PC-3 cell growth was examined using an MTT assay method and cell number was calculated by means of a standard regression line. The expressions of MUC-1 and integrins α2, α5, and β 1 were detected using FACScan flow cytometric analysis. Levels of MUC-1 mRNA were determined using reverse transcriptase PCR (RT-PCR). RESULTS. Treatment of PC-3 cells with arctiin decreased the cell number in a concentration- and time-dependent manner in serum-containing condition. Arctiin preferentially induced cell detachment, but did not have anti-proliferation or cytotoxic effects in PC-3 cells. The arctiin-induced effect was inhibited by cycloheximide, indicating that protein synthesis was required. FACScan flow cytometric analysis demonstrated that arctiin increased the expression of the anti-adhesion mucin MUC-1, but did not affect integrin expression in PC-3 cells. The arctiin-induced increase in MUC-1 protein expression was due to up-regulation of mRNA, as revealed by RT-PCR analysis. CONCLUSIONS. Arctiin significantly induces cell detachment and decreases the cell numbers via the up-regulation of MUC-1 mRNA and protein in PC-3 cells. ? 2003 Wiley-Liss, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-1942475772&doi=10.1002%2fpros.10364&partnerID=40&md5=f2f02f6a90acf36d9f3777656f54cb4b https://scholars.lib.ntu.edu.tw/handle/123456789/575506 |
ISSN: | 0270-4137 | DOI: | 10.1002/pros.10364 | SDG/關鍵字: | alpha2 integrin; alpha5 integrin; arctiin; beta1 integrin; mucin 1; article; cancer growth; cancer inhibition; cell adhesion; cell count; cell growth; controlled study; flow cytometry; fluorescence activated cell sorting; human; human cell; male; nitroblue tetrazolium test; priority journal; prostate cancer; protein expression; protein function; regulatory mechanism; reverse transcription polymerase chain reaction; upregulation; CA-15-3 Antigen; Cell Adhesion; Drugs, Chinese Herbal; Flow Cytometry; Furans; Gene Expression Regulation, Neoplastic; Glucosides; Humans; Kinetics; Male; Prostatic Neoplasms; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Tumor Cells, Cultured; Up-Regulation |
顯示於: | 醫學系 |
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