https://scholars.lib.ntu.edu.tw/handle/123456789/575514
標題: | Induction of mitotic arrest and apoptosis in human prostate cancer PC-3 cells by evodiamine | 作者: | Huang D.-M. JIH-HWA GUH Huang Y.-T. SHIH-CHIEH CHUEH Chiang P.-C. Teng C.-M. |
公開日期: | 2005 | 出版社: | Lippincott Williams and Wilkins | 卷: | 173 | 期: | 1 | 起(迄)頁: | 256-261 | 來源出版物: | Journal of Urology | 摘要: | Purpose: Although evodiamine, an alkaloid isolated from Evodiae fructus, has been reported to exert anticancer activities, to our knowledge its target and mechanism of action have not yet been explored. We examined the anticancer activities and action mechanism of evodiamine. Materials and Methods: Human prostate cancer PC-3 cells were used in this study. The cytotoxic effect and cell growth inhibition were examined using the MTT (3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide) assay and sulforhodamine B assay, respectively. The apoptotic effect was determined using TUNEL assay and the progression of cells through the cell cycle and cell apoptosis were examined by FACScan flow cytometry (Becton Dickinson, Sunnyvale, California). In situ mitotic spindle detection and in vitro tubulin polymerization assay were performed by immunofluorescence staining for β-tubulin and CytoDYNAMIX ScreenTM3 (CDS-03) kits (Cytoskeleton, Denver, Colorado). Results: It was found that treatment of PC-3 cells with evodiamine decreased the cell number in a concentration and time dependent manner, and effectively inhibited PC-3 cell growth via the induction of cell cycle arrest at the G2/M phase and subsequent apoptosis. In an in situ assay we found that evodiamine inhibited microtubule spindle formation. In a cell-free assay system of tubulin polymerization evodiamine inhibited the polymerization of microtubules in a concentration dependent manner. Conclusions: These data suggest that evodiamine shows anticancer activity through inhibition of tubulin polymerization. This antitubulin activity might make evodiamine a potential anticancer drug. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-10344234253&doi=10.1097%2f01.ju.0000141587.72429.e3&partnerID=40&md5=f09b43936b12c4873f555e37c224cc49 https://scholars.lib.ntu.edu.tw/handle/123456789/575514 |
ISSN: | 0022-5347 | DOI: | 10.1097/01.ju.0000141587.72429.e3 | SDG/關鍵字: | 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; antineoplastic agent; beta tubulin; evodiamine; sulforhodamine B; antineoplastic activity; apoptosis; article; cell count; cell cycle; cell growth; concentration response; controlled study; cytotoxicity; device; disease course; drug mechanism; growth inhibition; human; human cell; human tissue; immunofluorescence; microtubule assembly; mitosis inhibition; mitosis spindle; nick end labeling; priority journal; prostate cancer |
顯示於: | 醫學系 |
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