https://scholars.lib.ntu.edu.tw/handle/123456789/575753
標題: | Satellite-like gold nanocomposites for targeted mass spectrometry imaging of tumor tissues | 作者: | Tseng Y.-T Harroun S.G Wu C.-W Mao J.-Y Chang H.-T Huang C.-C. HUAN-TSUNG CHANG |
關鍵字: | Aptamers; Gold nanocomposites; Laser desorption/ionization mass spectrometry; Self-assembly; Tissue imaging | 公開日期: | 2017 | 卷: | 1 | 期: | 2 | 起(迄)頁: | 141-153 | 來源出版物: | Nanotheranostics | 摘要: | We have developed a simple, rapid, high-throughput cancer diagnosis system using functional nanoparticles (NPs) consisting of poly(catechin) capped-gold NPs (Au@PC NPs) and smaller nucleolin-binding aptamer (AS1411) conjugated gold NPs (AS1411–Au NPs). The AS1411–Au NPs/Au@PC NP is used as a targeting agent in laser desorption/ionization mass spectrometry (LDI-MS)-based tumor tissue imaging. Self-assembled core-shell Au@PC NPs are synthesized by a simple reaction of tetrachloroaurate(III) with catechin. Au@PC NPs with a well-defined and dense poly(catechin) shell (~40?60 nm) on the surface of each Au core (~60?80 nm) are obtained through careful control of the ratio of catechin to gold ions, as well as the pH of the reaction solution. Furthermore, we have shown that AS1411-conjugated Au NPs (13-nm) self-assembled on Au@PC NP can from a satellite-like gold nanocomposite. The high density of AS1411–Au NPs on the surface of Au@PC NP enhances multivalent binding with nucleolin molecules on tumor cell membranes. We have employed LDI-MS to detect AS1411–Au NPs/Au@PC NPs labeled nucleolin-overexpressing MCF-7 breast cancer cells through the monitoring of Au cluster ions ([Aun]+; 1 ? n ? 3). The ultrahigh signal amplification from Au NPs through the formation of a huge number of [Aun ]+ ions results in a sensing platform with a limit of detection of 100 MCF-7 cells mL?1. Further, we have applied the satellite-like AS1411–Au NPs/Au@PC NP nanocomposite as a labeling agent for tumor tissue imaging by LDI-MS. Our nanocomposite-assisted LDI-MS imaging platform can be extended for simultaneous analysis of different tumor markers on cell membranes when using different ligand-modified metal nanoparticles. ? Ivyspring International Publisher. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85051127675&doi=10.7150%2fntno.18897&partnerID=40&md5=004d0e6febc20d46ea7c35ee8fcbfd2e https://scholars.lib.ntu.edu.tw/handle/123456789/575753 |
ISSN: | 22067418 | DOI: | 10.7150/ntno.18897 |
顯示於: | 化學系 |
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