https://scholars.lib.ntu.edu.tw/handle/123456789/576289
標題: | CO2-Responsive Water-Soluble Conjugated Polymers for in Vitro and in Vivo Biological Imaging | 作者: | Cheng C.-C Lai Y.-C Shieh Y.-T Chang Y.-H Lee A.-W Chen J.-K Lee D.-J Lai J.-Y. DUU-JONG LEE |
關鍵字: | Amines; Biocompatibility; Carbon dioxide; Fluorescence; Hydrophilicity; Hydrophobicity; Phase separation; Surface charge; Enhanced fluorescence; Fluorescence enhancement; Fluorescence intensities; Hydrophilic and hydrophobic; Polymeric structures; Spherical structures; Switching properties; Water-soluble conjugated polymers; Conjugated polymers; carbon dioxide; nanoparticle; nitrogen; polymer; animal experiment; aqueous solution; Article; carbon nuclear magnetic resonance; cell membrane; cell viability; confocal laser scanning microscopy; conjugation; controlled study; cytotoxicity; dispersity; embryo; female; flow cytometry; fluorescence; HeLa cell line; human; human cell; in vitro study; in vivo study; matrix assisted laser desorption ionization time of flight mass spectrometry; molecular weight; NIH 3T3 cell line; nonhuman; particle size; photoluminescence; photon correlation spectroscopy; polymerization; priority journal; proton nuclear magnetic resonance; protonation and deprotonation; scanning electron microscopy; surface charge; surface property; water solubility; zebra fish | 公開日期: | 2020 | 卷: | 21 | 期: | 12 | 起(迄)頁: | 5282-5291 | 來源出版物: | Biomacromolecules | 摘要: | Water-soluble conjugated polymers (WCPs) composed of a hydrophobic polythiophene main chain with hydrophilic tertiary amine side-chains can directly self-assemble into sphere-like nano-objects in an aqueous solution due to phase separation between the hydrophilic and hydrophobic segments of the polymeric structure. Due to the presence of gas-responsive tertiary amine moieties in the spherical structure, the resulting polymers rapidly and reversibly tune their structural features, surface charge, and fluorescence performance in response to alternating carbon dioxide (CO2) and nitrogen (N2) bubbling, which leads to significantly enhanced fluorescence and surface charge switching properties and a stable cycle of on and off switching response. In vitro studies confirmed that the CO2-treated polymers exhibited extremely low cytotoxicity and enhanced cellular uptake ability in normal and tumor cells, and thus possess significantly improved fluorescence stability, distribution, and endocytic uptake efficiency within cellular organisms compared to the pristine polymer. More importantly, in vivo assays demonstrated that the CO2-treated polymers displayed excellent biocompatibility and high fluorescence enhancement in living zebrafish, whereas the fluorescence intensity and stability of zebrafish incubated with the pristine polymer decreased linearly over time. Thus, these CO2 and N2-responsive WCPs could potentially be applied as multifunctional fluorescent probes for in vivo biological imaging. ? 2020 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096820507&doi=10.1021%2facs.biomac.0c01336&partnerID=40&md5=b79c02cea8d751c30df3139436391e9c https://scholars.lib.ntu.edu.tw/handle/123456789/576289 |
ISSN: | 15257797 | DOI: | 10.1021/acs.biomac.0c01336 |
顯示於: | 化學工程學系 |
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