https://scholars.lib.ntu.edu.tw/handle/123456789/577061
標題: | A manual and portable centrifuge combined with a paper-based immunoassay for myocardial infarction diagnosis | 作者: | Yuan H Tsai T.-T Wang H.-P Chien Y.-S Chen C.-A Chu C.-C Ho C.-T Chu P.-H CHIEN-FU CHEN |
關鍵字: | Analytic equipment; Cardiology; Diagnosis; Detection limits; Detection process; Early diagnosis; Enzyme linked immunosorbent assay; Mi diagnosis; Mortality rate; Myocardial Infarction; Plasma separation; Centrifuges | 公開日期: | 2021 | 卷: | 409 | 來源出版物: | Chemical Engineering Journal | 摘要: | Myocardial infarction (MI) causes hundreds of thousands of deaths annually. Early diagnosis of MI by detecting the concentration of troponin I in plasma can help limit mortality rates. However, plasma separation and early diagnosis are particularly difficult to achieve in resource-limited settings. In this study, we demonstrate a manual and portable centrifuge along with a paper analytical device to measure troponin I for early MI diagnosis. With this so-called “Handfuge,” pure plasma can be achieved with a yield of up to 47.1% and purity as high as 99.9% in less than 3 min without electricity. The resulting plasma can then be directly used for detecting troponin I using a paper-based enzyme-linked immunosorbent assay (ELISA), with a detection limit as low as 0.03 ng/mL. The whole detection process consumes just 10 ?L of blood, which is similar to the volume obtained by a clinical finger-prick. The proposed centrifuge together with the paper analytical device possesses the potential to replace existing techniques in troponin I detection for early MI diagnoses in resource-limited regions of the world. ? 2020 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098193103&doi=10.1016%2fj.cej.2020.128131&partnerID=40&md5=6cb6b00aebed95127f5b0b8cb730c46f https://scholars.lib.ntu.edu.tw/handle/123456789/577061 |
ISSN: | 13858947 | DOI: | 10.1016/j.cej.2020.128131 |
顯示於: | 應用力學研究所 |
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