https://scholars.lib.ntu.edu.tw/handle/123456789/577133
標題: | In vitro differentiation of human placenta-derived multipotent cells into schwann-like cells | 作者: | Juan C.-H Chen M.-H Lin F.-H Wong C.-S Chien C.-C Chen M.-H. Lin, Feng-Huei |
關鍵字: | Differentiation; Peripheral nerve; Placenta-derived multipotent stem cell; Schwann cell | 公開日期: | 2020 | 卷: | 10 | 期: | 12 | 起(迄)頁: | 1-12 | 來源出版物: | Biomolecules | 摘要: | Human placenta-derived multipotent stem cells (PDMCs) resembling embryonic stem cells can differentiate into three germ layer cells, including ectodermal lineage cells, such as neurons, astrocytes, and oligodendrocytes. The favorable characteristics of noninvasive cell harvesting include fewer ethical, religious, and legal considerations as well as accessible and limitless supply. Thus, PDMCs are attractive for cell-based therapy. The Schwann cell (SC) is the most common cell type used for tissue engineering such as nerve regeneration. However, the differentiation potential of human PDMCs into SCs has not been demonstrated until now. In this study, we evaluated the potential of PDMCs to differentiate into SC-like cells in a differentiation medium. After induction, PDMCs not only exhibited typical SC spindle-shaped morphology but also expressed SC markers, including S100, GFAP, p75, MBP, and Sox 10, as revealed by immunocytochemistry. Moreover, a reverse transcription-quantitative polymerase chain reaction analysis revealed the elevated gene expression of S100, GFAP, p75, MBP, Sox-10, and Krox-20 after SC induction. A neuroblastoma cell line, SH-SY5Y, was cultured in the conditioned medium (CM) collected from PDMC-differentiated SCs. The growth rate of the SH-SY5Y increased in the CM, indicating the function of PDMC-induced SCs. In conclusion, human PDMCs can be differentiated into SC-like cells and thus are an attractive alternative to SCs for cell-based therapy in the future. ? 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097381755&doi=10.3390%2fbiom10121657&partnerID=40&md5=8292de42da43e8304ca1fd1fe9bf389e https://scholars.lib.ntu.edu.tw/handle/123456789/577133 |
ISSN: | 2218273X | DOI: | 10.3390/biom10121657 | SDG/關鍵字: | glial fibrillary acidic protein; glyceraldehyde 3 phosphate dehydrogenase; myelin basic protein; nerve growth factor receptor; protein S 100; transcription factor; biological marker; early growth response factor 2; EGR2 protein, human; fibroblast growth factor 2; forskolin; GFAP protein, human; glial fibrillary acidic protein; heregulin beta1; myelin basic protein; neu differentiation factor; platelet derived growth factor; platelet-derived growth factor A; protein S 100; PSIP1 protein, human; signal transducing adaptor protein; SOX10 protein, human; transcription factor; transcription factor Sox; adipogenesis; Article; cell differentiation; cell structure; cell therapy; controlled study; flow cytometry; gene expression; growth rate; human; human cell; immunocytochemistry; immunofluorescence; immunophenotyping; in vitro study; multipotent stem cell; nerve regeneration; peripheral nerve; placenta; placenta function; polymerase chain reaction; quantitative analysis; regenerative medicine; Schwann cell; SH-SY5Y cell line; tissue engineering; cell differentiation; cell proliferation; chemistry; conditioned medium; cytology; drug effect; female; genetics; metabolism; multipotent stem cell; nerve cell; pharmacology; placenta; pregnancy; primary cell culture; Schwann cell; tumor cell line; Adaptor Proteins, Signal Transducing; Biomarkers; Cell Differentiation; Cell Line, Tumor; Cell Proliferation; Colforsin; Culture Media, Conditioned; Early Growth Response Protein 2; Female; Fibroblast Growth Factor 2; Gene Expression; Glial Fibrillary Acidic Protein; Humans; Multipotent Stem Cells; Myelin Basic Protein; Neuregulin-1; Neurons; Placenta; Platelet-Derived Growth Factor; Pregnancy; Primary Cell Culture; S100 Proteins; Schwann Cells; SOXE Transcription Factors; Transcription Factors |
顯示於: | 醫學工程學研究所 |
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