https://scholars.lib.ntu.edu.tw/handle/123456789/577161
標題: | Developing a glyoxal-crosslinked chitosan/gelatin hydrogel for sustained release of human platelet lysate to promote tissue regeneration | 作者: | Tsai C.-C Young T.-H Chen G.-S NAI-CHEN CHENG TAI-HORNG YOUNG |
公開日期: | 2021 | 卷: | 22 | 期: | 12 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | The clinical application of human platelet lysate (HPL) holds promise for tissue regen-eration, and the development of an efficient vehicle for its delivery is desired. Chitosan-based hydrogels are potential candidates, but they often exhibit weak mechanical properties. In this study, a chitosan/gelatin (CS-GE) hydrogel crosslinked by glyoxal was fabricated for sustained release of HPL. The influence of HPL on Hs68 fibroblast and human umbilical vein endothelial cell (HUVEC) culture was evaluated, and we found that supplementing 5% HPL in the medium could significantly improve cell proliferation relative to supplementing 10% fetal bovine serum (FBS). Moreover, HPL accelerated the in vitro wound closure of Hs68 cells and facilitated the tube formation of HUVECs. Subsequently, we fabricated CS-GE hydrogels crosslinked with different concentrations of glyoxal, and the release pattern of FITC-dextrans (4, 40 and 500 kDa) from the hydrogels was assessed. After an ideal glyoxal concentration was determined, we further characterized the crosslinked CS-GE hydrogels encapsulated with different amounts of HPL. The HPL-incorporated hydrogel was shown to significantly promote the proliferation of Hs68 cells and the migration of HUVECs. Moreover, the release pattern of transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor-BB (PDGF-BB) from hydrogel was examined in vitro, demonstrating a sustained release profile of the growth factors. Finally, the chick chorioallantoic membrane assay revealed that HPL encapsulation in the hydrogel significantly stimulated angiogenesis in ovo. These results demonstrate the great potential of the crosslinked CS-GE hydrogel to serve as an effective delivery system for HPL to promote tissue regeneration. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107881406&doi=10.3390%2fijms22126451&partnerID=40&md5=5948ef002efcf822ec947284c12b2454 https://scholars.lib.ntu.edu.tw/handle/123456789/577161 |
ISSN: | 16616596 | DOI: | 10.3390/ijms22126451 |
顯示於: | 醫學工程學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。