https://scholars.lib.ntu.edu.tw/handle/123456789/577192
標題: | Silver Nanoparticles on Nanoscale Silica Platelets (AgNP/NSP) and Nanoscale Silica Platelets (NSP) Inhibit the Development of Fusarium oxysporum f. sp. niveum | 作者: | Chang T.-H Liu Y.-W Lin Y.-H Lin J.-J Huang J.-W Hussain A Chang P.-F.L. JIANG-JEN LIN |
關鍵字: | Amino acids; Disease control; High resolution transmission electron microscopy; Metal nanoparticles; Nanostructured materials; Platelets; Scanning electron microscopy; Silica; Silver nanoparticles; Anti-fungal activity; Fluorescence staining; Fusarium oxysporums; Membrane permeability; Morphological effects; Nano scale; Scanning and transmission electron microscopy; watermelon; Fungi | 公開日期: | 2019 | 卷: | 2 | 期: | 11 | 起(迄)頁: | 4978-4985 | 來源出版物: | ACS Applied Bio Materials | 摘要: | Nanotechnology has attracted much attention recently because of its agricultural applications. In this study, we analyzed the ability of two potential nanomaterials (NMs), nanoscale silica platelets (NSP) and silver nanoparticles on nanoscale silica platelets (AgNP/NSP), to control Fusarium wilt [caused by Fusarium oxysporum f. sp. niveum (Fon)] disease in watermelon. Both AgNP/NSP and NSP significantly reduced Fon mycelial growth and spore viability. In addition, AgNP/NSP decreased the mycelium viability at concentrations of 150 and 200 ppm. Scanning and transmission electron microscopy showed significant morphological effects on Fon cells, such as increased roughness and interior hollowing after AgNP/NSP and NSP treatments. Further, fluorescence staining experiments showed that a concomitant increase in membrane permeability occurred after treatment with NMs. The biochemical effects of NM treatment included a significant reduction in secreted cellulase activity. Interestingly, the addition of cysteine as a reducing agent decreased effects of NSP on Fon spores, suggesting suppression of Fon spore development attributable to oxidative stress. Taken together, these results indicate that AgNP/NSP and NSP may potentially serve as nanofungicides for future control of Fusarium wilt and other fungal diseases. ? 2019 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075182348&doi=10.1021%2facsabm.9b00699&partnerID=40&md5=cc67bcb780600eeab92eb7787ca65f72 https://scholars.lib.ntu.edu.tw/handle/123456789/577192 |
ISSN: | 25766422 | DOI: | 10.1021/acsabm.9b00699 |
顯示於: | 高分子科學與工程學研究所 |
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