https://scholars.lib.ntu.edu.tw/handle/123456789/577428
標題: | PCL-Blended Chitosan Substrates for Patterning the Heterotypic Cell Distribution in an Epithelial and Mesenchymal Coculture System | 作者: | Li, Yi-Chen Ethan JYH-HORNG WANG Wang, Yu-Hsin Shao, Hung-Jen Young, Lu-Chieh TAI-HORNG YOUNG |
關鍵字: | HaCaT cells; Hs68 cells; blended substrates; chitosan; coculture; poly(ε-caprolactone) (PCL) | 公開日期: | 2020 | 卷: | 6 | 期: | 7 | 來源出版物: | ACS biomaterials science & engineering | 摘要: | Cell-cell and cell-substrate interactions in coculture systems are very important to the context of biomaterial scaffolds for tissue engineering applications. Understanding the cellular interactions and distribution of epithelial-mesenchymal microtissues on the controllable biomaterial surfaces is useful to study the organoid applications. The aim of the present study is to investigate the effects of chitosan/poly(ε-caprolactone) (PCL)-blended biomaterials on the distribution and spheroid formation of HaCaT and Hs68 cells in a coculture system. In this study, we demonstrated that the cocultured cells gradually changed their pattern from core/shell spheroid to monolayered morphology as the PCL content increased in the blended substrates. This indicates that the chitosan/PCL-blended substrates are able to regulate cell-substrate and cell-cell interactions to modify the distribution of HaCaT and Hs68 cells similar to various mesenchymal-epithelial organizations in biological tissues. Moreover, we also developed a two-dimension lattice model to elaborate the dependence of cell spheroid development on complex cell-cell interactions. This information may be helpful to develop appropriate biomaterials with appropriate properties to the applications of engineered epithelial-mesenchymal organoids. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/577428 | ISSN: | 2373-9878 | DOI: | 10.1021/acsbiomaterials.0c00304 |
顯示於: | 醫學系 |
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